Hotline:400-820-3792Inhibitors ? ScreeningLibraries ? Proteinswww.MedChemEDNH28Cat.No.:HY-175037分子式: C??H??ClF?N?O分子量: 436.9作用靶點(diǎn): NF-κB;p38MAPK;Bcl-2Family;Caspase;Apoptosis作用通路: NF-κB;MAPK/ERKPathway;Apoptosis儲存方式: PleasestoretheproductundertherecommendedconditionsintheCertificateofAnalysis.BIOLOGICALACTIVITY生物活性DNH28是一種強(qiáng)效的NF-κB和MAPK抑制劑，對HepG2細(xì)胞的IC50值為0.93μM。DNH28通過下調(diào)Bcl-2，上調(diào)BAX和C-caspase-3的表達(dá)，誘導(dǎo)細(xì)胞凋亡(apoptosis)。DNH28可阻滯細(xì)胞周期，抑制細(xì)胞遷移。DNH28可用于肝細(xì)胞癌(HCC)的研究[1]。IC50&TargetCaspase3BaxBcl-2NF-κB體外研究DNH28(Compound28)exhibitslowertoxicitytowardTHLE-3cells(IC50=18.16μM)[1].DNH28(0.46-1.84μM,24h)inhibitsHepG2cellsproliferationbyinducingapoptosisanddose-dependentlyinhibitsHepG2cellsintheG2/Mphase[1].DNH28(0.46-1.84μM,24h)inhibitsHepG2cellmigrationdose-dependently,anditcompletelyinhibitsthemigrationabilityataconcentrationof1.84μM[1].DNH28(1.84μM,24h)effectivelyinhibitsthenucleartranslocationofp65inducedbyTNF-α[1].DNH28(0.46-1.84μM,24h)exertsanti-HCCeffectsbyinhibitingtheactivationofNF-κBandMAPKsignalingpathways[1].ApoptosisAnalysis[1]CellLine:HepG2cellsConcentration:0.46,0.92and1.84μMIncubationTime:24hResult:Inducedapoptosisdose-dependently.Reached45.90%ofapoptoticcellsat1.84μM(comparedwith14.57%inthecontrol1/3 MasterofBioactiveMolecules—您身邊的抑制劑大師www.MedChemEgroup).CellCycleAnalysis[1]CellLine:HepG2cellsConcentration:0.46,0.92and1.84μMIncubationTime:24hResult:Reached76.22%G2/Mphaseratioat1.84μM.CellMigrationAssay[1]CellLine:HepG2cellsConcentration:0.46,0.92and1.84μMIncubationTime:24hResult:Partiallysuppressedcellmigration,whereasat1.84μM,thescratchwidthremainedunchanged.RT-PCR[1]CellLine:HepG2cellsConcentration:0.46,0.92and1.84μMIncubationTime:24hResult:Increasetheexpressionofpro-apoptoticmRNAlevelBAXandC-caspase-3.Reducetheexpressionoftheanti-apoptoticmRNAlevelBCL-2.WesternBlotAnalysis[1]CellLine:HepG2cellsConcentration:0.46,0.92and1.84μMIncubationTime:24hResult:Exhibiteddose-dependentinhibitionofthephosphorylationofp65,IκB-α,p38andERK.Significantlydown-regulatedtheactivationoftheNF-κBandMAPKsignalingpathwaysREFERENCES[1].YuL,etal.Novel3,4-dihydronaphthalen-1(2H)-onederivativespromoteapoptosisandinhibitmigrationofhepatocellularcarcinomacells2/3 MasterofBioactiveMolecules—您身邊的抑制劑大師www.MedChemEviainhibitionofNF-κBandMAPKsignalingpathways.EurJMedChem.2025Oct15;296:117898.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplication