20-R20R20RR8R7R2R3pKD-L-2L-280nm280nm-1-2Sanger-NH22,4-DNB-3Edman-NH2PIT-SangerEdmenN231010-NC-,3.60.54nm,-0.35nm, RR1. 1-62C3 2. 1.1248mol/L-2O2RpHpIpHpIpHpI1. 2. SDS-SDSSDSSDS3. SSS1. dipolarion2. essential amino acid3. (isoelectric point,pI)4. (rare amino acid)5. (nonprotein amino acid)6.(configuration)7.(protein primary structure)8. (conformation)9. (protein secondary structure)10. (domain)11. (protein tertiary structure)12. (hydrogen bond)13. (protein quaternary structure)14. (ionic bond)15. (super-secondary structure)16. (hydrophobic bond)17. ( van der Waals force)18. (salting out)19. (salting in)20. (denaturation)21. (renaturation)22. (precipitation)23. gel electrophoresis24. chromatography()1. _2. _%110mg,_%3. 20_2_.4. _3280nm5. pI10.76,pH7_6. 20_7. _8. -_,_-_9. -_10. _11. _12. _.13. _14. GSH_15. _,_,_16. pH_17. _18. _19. _20. 8.04.510.0,pH8.0_21. pH=pI_pHpI_22. pK1(-COOH)=2.19,pK2 (-NH+3 ) = 9.67,pKR(R)= 4.25,_23. _24. a90 000b45 000c110 000_25. 0.34%_.0.34%4_.26. -180_-_.() 1. pHA. B. C. D. E. 2.A. B. C. D. E. 3.280nmA. B. C. D. 4.4A. B. C. D. 5.A. B. C. D. 6.-A. B. C. 3.6D. 0.15nm.7.A. B. C. D. 8.A. LeuB. AlaC. GlyD. SerE. Val9.A. B. C. D. 10.A. B. C. D. 11.A. pHB. C. D. 12.A. B. C. D. 13.-A. B. C. D. E. 14.-A. -B. C. -D. 0.35nm15.A. B. C. D. 16.A. B. C. D. E. 17.A. B. C. D. 18. A. B. C. D. 19.A. B. C. D. pH20.A. B. C. D. E. 21.A. B. C. ABD. E. 22.A. B. C. D. A,BC() () 1.() 2.() 3.() 4.-() 5.D-L-() 6.() 7.20() 8.() 9.pI-COOH-NH2 () 10.() 11.() 12.() 13.FDNBEdmanN-() 14.20-() 15.R() 16.() 17.() 18.() 19.() 20.() 21.()22.()23. ()24.1. 2. 3. 4. 5. 6. 7. 8. 9. CNBr6mol/L HCl-12344510. 151015Ile-Ala-His-Thr-Tyr-Gly-Pro-Glu-Ala-Ala-Met-Cys-Lys-Try-Glu-Ala-Gln-202527Pro-Asp-Gly-Met-Glu-Cys-Ala-Phe-His-Arg1-231. 2. 3. pHpI4. 205. 6. 7. 8.