1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEPropidium IodideCat. No.: HY-D0815CAS No.: 25535-16-4Synonyms: PI分式: CHIN分量: 668.39作靶點: Others作通路: Others儲存式: 4C, protect from light* In solvent : -80C, 6 months; -20C, 1 month (protect fromlight)溶解性數(shù)據(jù)體外實驗 H2O : 6 mg/mL (8.98 mM

2、; Need ultrasonic and warming)Mass Solvent1 mg 5 mg 10 mg Concentration制備儲備液1 mM 1.4961 mL 7.4807 mL 14.9613 mL5 mM 0.2992 mL 1.4961 mL 2.9923 mL10 mM - - -請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液，并請注意儲備液的保存式和期限。BIOLOGICAL ACTIVITY物活性 Propidium Iodide可于細(xì)胞染的紅熒光染料。體外研究Propidium Iodide is a cell-membrane impermeab

3、le dye with characteristic excitation maximum at 535 nm andemission maximum at 617 nm which intercalates with nucleic acids with a stoichiometry of one dye per 4-5base pairs with little sequence preference. Propidium Iodide has evidenced of having no toxic effects onneurons, being todays most common

4、 marker for membrane integrity and cell viability when applied prior tofixation (pre-fixation Propidium Iodide staining method). The pre-fixation staining has been widely used for1/2 Master of Small Molecules 您邊的抑制劑師www.MedChemEquantitative assessments of neuronal cell decline in models of acute neu

5、rodegeneration, visualized asintensely labeled PI+-pycnotic nuclei of degenerating neurons 1. Propidium Iodide cannot cross themembrane of live cells, making it useful to measure the percentage of apoptotic cells by flow-cytometricanalysis. The flow cytometric data shows an excellent correlation wit

6、h the results obtained with bothelectrophoretic and colorimetric methods. This new rapid, simple and reproducible method proves useful forassessing apoptosis of specific cell populations in heterogeneous tissues such as bone marrow, thymus andlymph nodes 2.PROTOCOLCell Assay 2 Flow cytometric analys

7、is: Propidium iodide is prepared in in 0.1% sodium citrate plus 0.1% Triton X-100 (50 g/mL). The 200 g centrifuged cell pellet is gently resuspended in 1.5 mL hypotonlc fluorochrome solution(Propidium iodide 50 g/mL), in 1275 polypropylene tubes. The tubes are placed at 4C in the darkovernight befor

8、e the flow cytometric analysis. The propidium Iodide fluorescence of individual nuclei ismeasured using a FACScan flow cytometer. The nuclei traverses the light beam of a 488 nm Argon laser. A560 nm dichrolc nurror (DM 570) and a 600 nm band pass filter (bandwidth 35 nm) are used for collecting ther

9、ed fluorescence due to propidium Iodide staining of DNA and the data are registered on a logarithmic scale2.MCE has not independently confirmed the accuracy of these methods. They are for reference only.戶使本產(chǎn)品發(fā)表的科研獻(xiàn) Mol Med Rep. 2019 Jan;19(1):41-50. Aging (Albany NY). 2018 Nov 28;10(11):3353-3370. C

10、hinese Pharmacological Bulletin. 2018 May; 34(5): 620-626.See more customer validations on HYPERLINK / www.MedChemEREFERENCES1. Hezel M, et al. Propidium iodide staining: a new application in fluorescence microscopy for analysis of cytoarchitecture in adult anddeveloping rodent brain. Micron. 2012 Oct;43(10):1031-8.2. A rapid and simple method for measuring thymocyte apoptosis by propidium iodidestaining and flow cytometry. J Immunol Methods.1991 Jun 3;139(2):271-9.McePdfHeightCaution: Product has not been f