1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEL-HomocystineCat. No.: HY-W011690CAS No.: 626-72-2分式: CHNOS分量: 268.35作靶點: Endogenous Metabolite作通路: Metabolic Enzyme/Protease儲存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實驗 DMSO : 1 mg/mL (3.73 mM

2、; ultrasonic and adjust pH to 5 with HCl)H2O : 1 mg/mL (3.73 mM; ultrasonic and adjust pH to 2 with HCl)Mass Solvent1 mg 5 mg 10 mg Concentration制備儲備液1 mM 3.7265 mL 18.6324 mL 37.2648 mL5 mM - - -10 mM - - -請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液，并請注意儲備液的保存式和期限。BIOLOGICAL ACTIVITY物活性 L-HomocystineL-homocystein

3、e 的種氧化物，L-homocysteine 種抗栓因，管舒張損害劑，促炎因，也 研究管疾病發(fā)機制的內(nèi)質(zhì)應(yīng)激反應(yīng)的誘導(dǎo)劑。IC50 & Target Human Endogenous Metabolite體內(nèi)研究A single or multiple doses of L-Homocystine administered to mice during organogenesis can aggravate the1/2 Master of Small Molecules 您邊的抑制劑師www.MedChemEdevelopmental disturbances caused by a sin

4、gle dose of VPA administered on GD 8. Whereas, VPA lowerssignificantly plasma FA and vitamin B12 concentrations, it has no direct impact on the homocysteineconcentrations. Therefore, it is proposed that high levels of homocysteine disturb the FA, vitamin B12, andpossibly methionine metabolism thus p

5、roviding a favorable situation for VPA to interfere with thedevelopment of susceptible embryos 1.PROTOCOLAnimal Mice 1Administration 1 Groups of mice are first injected on GD 8 with a single dose of 75 mg/kg of L-Homocystine or an equalvolume of saline. One half of the L-Homocystine-treated animals

6、then receive a single dose of 600 mg/kg ofVPA (H-HoCys-OH)2+VPA group), while the other half are injected with a proportionate volume of saline (L-Homocystine+saline group). In the other experiment, mice are treated with a daily dose of 75 mg/kg of L-Homocystine or a proportionate volume of saline s

7、tarting from GD 5 and continue through GD 10. One half ofthe L-Homocystine-treated animals also have a single exposure to 600 mg/kg of VPA (L-Homocystine+VPAgroup) or a proportionate volume of saline (L-Homocystine+saline group) on GD 8. The total volume of fluidinjected corresponded to the body wei

8、ght and does not exceed 0.45 mL 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Padmanabhan R, et al. Effect of maternal exposure to homocystine on sodium valproate-induced neural tube defects in the mouseembryos. Eur J Nutr. 2006 Sep;45(6):311-9McePdfHeightCaution: Product has not been fully validated for medical application