1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEThiamine nitrateCat. No.: HY-B2223CAS No.: 532-43-4Synonyms: Vitamin B1 nitrate分式: CHNOS分量: 327.36作靶點(diǎn): Others作通路: Others儲(chǔ)存式: 4C, protect from light* In solvent : -80C, 6 months; -20C, 1 month (protect fromlight)溶解性數(shù)據(jù)體外實(shí)驗(yàn) DMSO :

2、15 mg/mL (45.82 mM; Need ultrasonic and warming)Mass Solvent1 mg 5 mg 10 mg Concentration制備儲(chǔ)備液1 mM 3.0547 mL 15.2737 mL 30.5474 mL5 mM 0.6109 mL 3.0547 mL 6.1095 mL10 mM 0.3055 mL 1.5274 mL 3.0547 mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲(chǔ)備液，并請(qǐng)注意儲(chǔ)備液的保存式和期限。BIOLOGICAL ACTIVITY物活性 Thiamine nitrate必不可少的維素，能增強(qiáng)正常的神經(jīng)元活

3、性。體外研究Thiamine levels in the blood of homozygous KO and KI mice fed a conventional diet are decreased to0.0580.051 and 0.1260.092 M, respectively, at 7 weeks compare to WT mice (0.7960.259 M). WhenWT and homozygous KO and KI mice are fed a Thiamine-restricted diet (Thiamine: 0.60 mg/100 g food),bloo

4、d Thiamine concentration at 5 and 14 days is markedly decreased to 0.0100.009 and 0.0100.006 M,respectively, compare to WT mice (0.6090.288 M). Thiamine concentration in brain homogenate of WT1/2 Master of Small Molecules 您邊的抑制劑師www.MedChemEmice fed a conventional diet is 3.812.18 nmol/g wet weight,

5、 and that of KO and KI is 1.330.96 and2.161.55 nmol/g wet weight, respectively. Notably, Thiamine concentration in brain homogenate decreasessteadily in KO and KI mice fed a thiamine-restrict diet (Thiamine: 0.60 mg/100 g food) for 5 days (0.950.72nmol/g wet weight) and 14 days (1.110.24 nmol/g wet

6、weight), respectively, compare to WT (3.651.02nmol/g wet weight), before the mice presenting an phenotype of disease 2.體內(nèi)研究 WT, homozygous, and heterozygous KO and KI mice fed a conventional diet (thiamine: 1.71 mg/100 g)survive for over 6 months without any phenotype of disease. Homozygous KO and K

7、I mice fed a Thiamine-restricted diet (thiamine: 0.60 mg/100 g food) show paralysis, weight loss, and immobility, and die within 12and 30 days, respectively. Similarly, homozygous KO and KI mice fed a Thiamine-restricted diet with an evenlower percentage of Thiamine (Thiamine: 0.27 mg/100 g food) di

8、e within 14 and 18 days, respectively.However, WT and heterozygous KO and KI mice fed a Thiamine-restricted diet (Thiamine: 0.60 mg or 0.27mg/100g food) survive for over 6 months without any phenotype of disease 2.PROTOCOLAnimal Slc19a3 E314Q KI mice are maintained routinely with conventional diet,

9、which has a Thiamine concentrationAdministration 2 (thiamine hydrochloride, MW=337.3) of 1.71 mg/100 g food. Two types of Thiamine-restrict food base on“purified diets for laboratory rodents” are prepared, in which Thiamine concentration is 0.60 mg/100 g food(35% Thiamine of conventional food) or 0.

10、27 mg/100 g food (16% Thiamine of conventional food). A high-Thiamine-containing food is also prepared from AIN-93M, in which Thiamine concentration is five times thatof CE-2 (thiamine: 8.50 mg/100 g food) 2.MCE has not independently confirmed the accuracy of these methods. They are for reference on

11、ly.REFERENCES1. Kenneth Osiezagha, et al.Thiamine Deficiency and Delirium. Innov Clin Neurosci. 2013 Apr; 10(4): 26-32.2. Kaoru Suzuki, et al. High-dose thiamine prevents brain lesions and prolongs survival ofSlc19a3-deficient mice. PLoS One. 2017; 12(6):e0180279.McePdfHeightCaution: Product has not be