1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEGinsenoside Rk1Cat. No.: HY-N2515CAS No.: 494753-69-4分式: CHO分量: 767作靶點(diǎn): NF-B; PI3K; JAK; Apoptosis作通路: NF-B; PI3K/Akt/mTOR; Epigenetics; JAK/STAT Signaling;Stem Cell/Wnt; Apoptosis儲(chǔ)存式: Please store the product under the recommen

2、ded conditions inthe COA.BIOLOGICAL ACTIVITY物活性 Ginsenoside Rk1 參皂苷 Rk1參的溫加提取物。Ginsenoside Rk1 具有抗炎作，抑制 Jak2/Stat3信號(hào)通路和 NF-B 的激活。Ginsenoside Rk1 具有抗腫瘤作，抗板聚集活性，抗炎作，抗胰島素抵抗，腎保護(hù)作，抗 作，認(rèn)知功能增強(qiáng)，脂質(zhì)積聚減少和預(yù)防質(zhì)疏松癥。Ginsenoside Rk1 通過(guò)觸發(fā)細(xì)胞內(nèi)活性氧 (ROS) 成和阻斷 PI3K/Akt 途徑誘導(dǎo)細(xì)胞凋亡。體外研究Ginsenoside Rk1 (0-40 M; 6 hours) inhibi

3、ts MCP-1 and TNF- mRNA induced by lipopolysaccharide (LPS),expression of IL-1 is inhibited at 40 M 2.Ginsenoside Rk1 (0-40 M; 24 hours) inhibits phosphorylation ofJAK2 and STAT3 (Tyr705 and Ser727) in LPS-induced RAW264.7 cells in a dose-dependent manner2.Ginsenoside Rk1 (0-160 M; 48 hours) results

4、in cell viability significant decrease 75.52 2.51% (40 M),52.72 2.54% (80 M), 17.41 2.94% (120 M)and 12.63 3.24% (160 M) compared with control3.Ginsenoside Rk1 (0-120 M; 24 hours) increases G0/G1 phase proportion accompanied with S and G2/Mphase proportion decrease in MDA-MB-231 cells 3.Ginsenoside

5、Rk1 (0-120 M; 24 hours) promotes thepercentage of apoptotic cells in a dose-dependent manner, exhibits to reduction of cell number with nucleusfragmentation, condensation and apoptotic body formation 3.RT-PCR 2Cell Line: RAW264.7 cellsConcentration: 10 M, 20 M, 40 MIncubation Time: 6 hours1/3 Master

6、 of Small Molecules 您邊的抑制劑師www.MedChemEResult: Inhibited JAK2-dependent STAT3 activation in LPS-activated RAW264.7 cells.Western Blot Analysis 2Cell Line: RAW264.7 cellsConcentration: 10 M, 20 M, 40 MIncubation Time: 6 hoursResult: Inhibited JAK2-dependent STAT3 activation in LPS-activated RAW264.7

7、cellsCell Viability Assay 3Cell Line: MDA-MB-231 cellsConcentration: 0 M, 40 M, 80 M, 120 MIncubation Time: 48 hoursResult: Inhibited MDA-MB-231 cells proliferation in a dose- and time-dependent manner.Cell Cycle Analysis 3Cell Line: MDA-MB-231 cellsConcentration: 0 M, 40 M, 80 M, 120 MIncubation Ti

8、me: 24 hoursResult: Induced G0/G1 phase arrest.Apoptosis Analysis 3Cell Line: MDA-MB-231 cellsConcentration: 0 M, 40 M, 80 M, 120 MIncubation Time: 24 hoursResult: Induced apoptosis in MDA-MB-231 cells.REFERENCES1. Elshafay A, et al. Ginsenoside Rk1 bioactivity: a systematic review. PeerJ. 2017 Nov

9、17;5:e3993.2. Yu Q,et al. Ginsenoside Rk1 suppresses pro-inflammatory responses in lipopolysaccharide-stimulated RAW264.7 cells by inhibiting theJak2/Stat3 pathway. Chin J Nat Med. 2017 Oct;15(10):751-757.3. Hong Y, et al. Ginsenoside Rk1 induces cell cycle arrest and apoptosis in MDA-MB-231 triple negative breast cancer cells. Toxicology.2/3 Master of Small Molecules 您邊的抑制劑師www.MedChemE2019 Apr 15;418:22-31.McePdfHeightCaution: Product has not been fully validated for