1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEL-TyrosineCat. No.: HY-N0473CAS No.: 60-18-4分式: CHNO分量: 181.19作靶點(diǎn): Endogenous Metabolite作通路: Metabolic Enzyme/Protease儲(chǔ)存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實(shí)驗(yàn) 1M HCl : 50 mg/mL (275.95 mM;

2、Need ultrasonic)DMSO : 1 mg/mL (insoluble or slightly soluble)Mass Solvent1 mg 5 mg 10 mg Concentration制備儲(chǔ)備液1 mM 5.5191 mL 27.5953 mL 55.1907 mL5 mM 1.1038 mL 5.5191 mL 11.0381 mL10 mM 0.5519 mL 2.7595 mL 5.5191 mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲(chǔ)備液，并請(qǐng)注意儲(chǔ)備液的保存式和期限。BIOLOGICAL ACTIVITY物活性 L-Tyrosine種必需氨 酸，能抑

3、制后部層中檸檬酸合成酶的活性。IC50 & Target Human Endogenous Metabolite體外研究Results show that L-Tyrosine in vitro inhibits citrate synthase activity in the posterior cortex (2.0 and 4.0 mM),malate dehydrogenase is not altered by L-Tyrosine and succinate dehydrogenase is increased in the1/2 Master of Small Molecules

4、 您邊的抑制劑師www.MedChemEposterior cortex (0.1, 1.0, 2.0 and 4.0 mM), hippocampus (1.0, 2.0 and 4.0 mM), striatum (4.0 mM) and liver(0.1, 1.0, 2.0 and 4.0 mM). When complex I activity is analyzed, inhibition is observed in hippocampus (4.0mM). In addition to inhibition in the hippocampus, complex II also

5、 is inhibited in the posterior cortex (0.1, 1.0,2.0 and 4.0 mM) and liver (1.0, 2.0 and 4.0 mM). For complex IIIII, activity is not altered by L-Tyrosine, andcomplex IV activity has decreased in the posterior cortex (1.0, 2.0 and 4.0 mM) following treatment with L-Tyrosine 1.體內(nèi)研究 The acute administr

6、ation of L-Tyrosine inhibits the activity of citrate synthase in the posterior cortex and liver;however, in the striatum, the activity is increased. The results also demonstrate that acute administration ofL-Tyrosine inhibits malate dehydrogenase and complex II, IIIII and IV of the mitochondrial res

7、piratory chainactivity in the posterior cortex and liver of rats. The succinate dehydrogenase enzyme and complex I activityare inhibited in the posterior cortex and increased in the striatum. Furthermore, energy metabolism in thehippocampus is not amended by an acute administration of L-Tyrosine 1.P

8、ROTOCOLKinase Assay 1 Posterior cortex, hippocampus, striatum and liver supernatants of 30-day-old rats are pre-incubated for 30min at 30C in the presence of L-Tyrosine (Tyr) at final concentrations ranging from 0.1, 1.0, 2.0 or 4.0 mM,and the activities of citrate synthase, malate dehydrogenase and

9、 respiratory chain complexes I, II, IIIII andIV are evaluated 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.Animal The equivalent of 500 mg/kg body weight of free L-Tyrosine is intraperitoneally administered in 30-day-oldAdministration 1 rats. Contr

10、ols receive in saline solution. About 1 h after injections, rats are killed by decapitation withoutanesthesia 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.戶使本產(chǎn)品發(fā)表的科研獻(xiàn) bioRxiv. 2019 Jan.See more customer validations on HYPERLINK / www.MedChemEREFERENCES1. Ferreira GK, et al. Effect of L-tyrosine in vitro and in vivo on energy metabolism parameters in brain and liver of young rats. NeurotoxRes. 2013 May;23(4):327-35.McePdfHeightCaution: Product has not been f