1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEProtocatechualdehydeCat. No.: HY-N0295CAS No.: 139-85-5Synonyms: Catechaldehyde; Protocatechuic aldehyde; Rancinamycin IV分式: CHO分量: 138.12作靶點(diǎn): Reactive Oxygen Species作通路: Immunology/Inflammation; Metabolic Enzyme/Protease; NF-B儲(chǔ)

2、存式: 4C, stored under nitrogen* In solvent : -80C, 6 months; -20C, 1 month (stored undernitrogen)溶解性數(shù)據(jù)體外實(shí)驗(yàn) DMSO : 50 mg/mL (362.00 mM)* means soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制備儲(chǔ)備液1 mM 7.2401 mL 36.2004 mL 72.4008 mL5 mM 1.4480 mL 7.2401 mL 14.4802 mL10 mM 0.72

3、40 mL 3.6200 mL 7.2401 mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲(chǔ)備液，并請(qǐng)注意儲(chǔ)備液的保存式和期限。體內(nèi)實(shí)驗(yàn)請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥式選擇適當(dāng)?shù)娜芙獍?，配制前?qǐng)先配制澄清的儲(chǔ)備液，再依次添加助溶劑(為保證實(shí)驗(yàn)結(jié)果的可靠性，體內(nèi)實(shí)驗(yàn)的作液，建議您現(xiàn)現(xiàn)配，當(dāng)天使；澄清的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；以下溶劑前的百分 指該溶劑在您配制終溶液中的體積占)：1. 請(qǐng)依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (18.10 mM); Clear solution

4、2. 請(qǐng)依序添加每種溶劑： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.5 mg/mL (18.10 mM); Clear solution3. 請(qǐng)依序添加每種溶劑： 10% DMSO 90% corn oil1/2 Master of Small Molecules 您邊的抑制劑師www.MedChemESolubility: 2.5 mg/mL (18.10 mM); Clear solutionBIOLOGICAL ACTIVITY物活性 Protocatechualdehyde (Catechaldehyde)從 Salviae M

5、iltiorrhizae 中分離出來的種天然多酚化合物，具有多種物活性，可作為抗氧化、抗衰、抗 消炎劑被泛應(yīng)于多個(gè)醫(yī)學(xué)領(lǐng)域 1。體外研究 Protocatechualdehyde (PCA) (50, 100 M, 24/48 hours ) treated MCF-7 cells significantly decrease cellgrowth by 11% and 20% in 24 hours and by 22% and 27% in 48 hours, respectively 2.Protocatechualdehyde (50, 100 M, 24 hours) treated

6、 MCF-7 cells are increased by 1.9-fold and 2.6-fold inthe concentrations of 50 M and 100 M, respectively. PCA suppresses proliferation of estrogen receptor(ER)-positive (MCF-7) breast cancer cells, but not ER-negative (MDA-MB-231) breast cancer cells 2.Protocatechualdehyde (0, 100, 200 M, 48 hours i

7、n HCT116 and SW480 cells) affects the enzyme activity ofHDAC and observed that PCA treatment resulted in inhibition of HDAC activity in dose-dependent manner3.Cell Proliferation Assay 2Cell Line: Human breast cancer cell (MCF-7 and MDA-MB-231)Concentration: 0, 5, 10, 25, 50, and 100 MIncubation Time

8、: 24, 48 hoursResult: Inhibited MCF-7 cells cell growth.Apoptosis Analysis 2Cell Line: Human breast cancer cell (MCF-7 and MDA-MB-231)Concentration: 0, 5, 10, 25, 50, and 100 MIncubation Time: 24, 48 hoursResult: Increased apoptosis in MCF-7 cells.REFERENCES1. Li S, et al. Evaluation of the Antibact

9、erial Effects and Mechanism of Action of Protocatechualdehyde against Ralstonia solanacearum.Molecules. 2016 Jun 9;21(6).2. Choi J, et al. Anticancer activity of protocatechualdehyde in human breast cancer cells. J Med Food. 2014 Aug;17(8):842-8.3. Jeong JB, et al. Protocatechualdehyde possesses anti-cancer activity through downregulating cyclin D1 and HDAC2 in human colorectalcancer cells. Biochem Biophys Res Commun. 2013 Jan 4;430(1):381-6.McePdfHeight2/2 Master of Small Molecules 您邊的抑制劑師www.MedChemECaution: Product has not been fully validated