1、Working concentrations and stock solutions.Working concentration:Stock solutionAntibioticsStrict controlWeak controlmg/ml:g/mldilutiong/mldilutionAmpicillin50(H 2O*)-20 o C200.4l/ml60-1251.2-2.5l/ml50-20 o CCarbenicillin200.4l/ml60-2001.2-4l/ml(H 2 O, 50%EtOH)34-20 o CChloramphenicol(EtOH, MetOH)250

2、.7l/ml1705l/ml10oD-cycloserine*20020l/ml(0.1M NaP pH8.0)+4 CGentamycin10(H 2O)-20 o C151.5l/mlHygromycin B10(H 2O)o101 l/ml40040l/ml-20 C+4 o C-Kanamicin50(H 2O)-20 o C100.2l/ml501l/mlKasugamycin10(H 2O)-20 o C1000100l/mlNalidixic acid5-20 o C153.0l/ml(H 2 O, pH to 11 /NaOH)Neomycin10(H 2O)-20 o C10

3、010l/ml80080l/mlRifampicin*34(MetOH)-20 o C1504.4l/mlSpectinomycin10(H 2O)-20 o C10010l/mlStreptomicin50(H 2O)-20 o C100.2l/ml501l/ml5 - 12.5-20 o C5010l/mlTetracycline*(EtOH, 70%EtOH)102 l/ml10020l/ml* - Unstable solution, prepare just before use;* - Light sensitive;* - Light sensitive, Mg2+ - inhi

4、bitor;Preparation of stock solutions.Ampicillin.ClearConc.1ml5ml10mlAmpicillin50mg/ml50mg250mg0.5gH 2 O1ml5ml10mlfilter sterilize; or prepare from the sterile water and the sterile powder;store at -20 o C in small aliquots (avoid more than 10 freezing-thawing cycles);it is possible to use 50% glycer

5、ol instead of H2 O. The advantage - solution is liquid at -20oC.some laboratories use 96% (or 50%) EtOH for ampicillin solubilization. There are twoadvantages in this method: (i) solution is liquid at -20oC, (ii) the solution is self sterilizing. But thedisadvantage is very serious: ethanol is a wea

6、k catalyst of ?-lactam ring disintegration. So,solution is unstable.Carbenicillin.ClearConc.1ml5ml10mlCarbenicillin50mg/ml50mg250mg0.5gH 2 O1ml5ml10mlfilter sterilize; or prepare from the sterile water and the sterile powder;store at -20 o C in small quantities (about 10 freezing-thawing cycles);200

7、g/ml for pETpfu.GE antibiotic mix (50 x).ClearConc.40mlCefotaxime 25g/ml25g/ml1mgCeftriaxone 25g/ml25g/ml1mgPotassium phosphate (pH 6.0) 0.1M40mlfilter sterilize; or prepare from the sterile KPO4 and the sterile powder;store at -20 0 C, in small quantities (about 10 freezing-thawing cycles).Tetracyc

8、line.ClearConc.1ml5ml10mlTetracycline5mg/ml5mg25mg50mgEtOH1ml5ml10mlsolution in EtOH, store at -20o C;Mg + - antagonist, for the selection it is necessary to use Mg-free medium.Chloramphenicol.ClearConc.1ml5ml10mlChloramphenicol34mg/ml34mg170mg340mgstore dry powder at 4o C, solution at -20o C (in Et

9、OH);BL21(DE3)pLyzS - 34g/ml - 1l for 1ml of medium.那是心與心的交匯，是相視的莞爾一笑，是一杯飲了半盞的酒，沉香在喉，甜潤在心。紅塵中，我們會相遇一些人，一些事，跌跌撞撞里，逐漸懂得了這世界，懂得如何經(jīng)營自己的內(nèi)心，使它柔韌，更適應(yīng)這風(fēng)雨征途，而不會在過往的錯失里糾結(jié)懊悔一生。時光若水，趟過歲月的河，那些舊日情懷，或溫暖或痛楚，總會在心中烙下深深淺淺的痕。生命是一座時光驛站，人們在那里來來去去。一些人若長亭古道邊的萋萋芳草，淪為泛泛之交；一些人卻像深山斷崖邊的幽蘭，只一株，便會馨香滿谷。人生，唯有品格心性相似的人，才可以在錦瑟華年里相遇相知，互為欣賞，互為懂得，并沉淀下來，做一生的朋友。試問，你的生命里，有無來過這樣一