1、小葉黃楊葉降血脂作用研究【摘要】目的研究小葉黃楊葉不同提取物的降血脂作用。方法選用系統(tǒng)溶媒(石油醚、醋酸乙酯、氯仿、正丁醇、水)的方法對(duì)小葉黃楊葉進(jìn)展提取，觀察各提取物對(duì)腹腔注射75%蛋黃乳液致高血脂癥模型小鼠血清總膽固醇及甘油三酯的影響。結(jié)果小葉黃楊葉醋酸乙酯提取物的高、低兩個(gè)劑量組均有顯著的降低血清總膽固醇和甘油三酯的作用，小葉黃楊葉的水提取物和石油醚提取物均有顯著的降低血清中總膽固醇的作用，小葉黃楊葉氯仿提取物及正丁醇提取物均無(wú)降血脂的作用。結(jié)論小葉黃楊葉醋酸乙酯提取物對(duì)高血脂癥小鼠有降血脂作用?！娟P(guān)鍵詞】小葉黃楊葉提取物降血脂Abstrat：bjetiveTstudytheantihy

2、perlipideiaeffetsfdiferentBuxusirphyllaleafextratsethdsDifferentextratsereextratedfrBuxusirphyllaleafbysysteatislvents(petrleubenzin，ethylaetate，hlrfr，n-butylalhlandaterrespetively)andtheantihyperlipideiaeffetsfdifferentextratserebservednserulevelsfttalhlesterlandtriglyerideinhyperlipideiadels，hiher

3、eestablishedthrugheliainjetinf75%egg-ylkeulsin.ResultsTheBuxusirphyllaleafethylaetateextrat(highdseandldse)hadbviuseffetnthelevelsfseruttalhlesterlandtriglyeride.rever，theeffetfhigh-dseethylaetateextratasbetterthanthetherextrats.Inadditin,theaterextratandn-butylalhlextratuldsignifiantlyredueseruttal

4、hlesterl,andthehlrfrextratandn-butylalhlextrathadnrearkableeffetnbldlipid.nlusinBuxusirphyllaleafethyl-aetateextratistheativepartfBuxusirphyllaleafinreduingbldlipid.Keyrds：BuxusirphyllaleafExtratsAntihyperlipideiaeffetBuxusirphyllaleafisthedryleaffrBuxusirphylla.(FailyGenus).Ardingtthe“pendiufateria

5、edia,Buxusirphyllaanprteflf“qiandbldirulatin,eliinatepathgenietness,dredgeandativatetheeridian.ylvirbuxineDisnekindfalkalidextratedfrtherhizefBuxusirphyllaandtherplantsbelngedtthesaeGenus1.Inreentyears,liniallyandexperientallyylvirbuxineDplaysagdrleinanti-arrythia,anti-yardialIsheiaandstrengtheningh

6、eart,andithasprtetivefuntinttheautebrainisheiabeauseitanpassthrughthebldbrainbarrier,suppressthefratinfthrbus,iprveiryle2-3,hever,untilnnpharalgialresearhreprtsntheBuxusirphyllaleafhasbeenseenintheliterature.Inthisexperientebservedtheeffetsfsysteslventextratfrbuxusirphyllaleafntheseruttalhlesterland

7、triglyerideinhyperlipideiadelie.1aterialsandethds1.1Drugs,Reagents,AnialsandInstruents1.1.1PreparatinfdrugsDryBuxusirphyllaleaves,frYngfu,GuangxiGuilin,ereextratedith70perentethanl.Thepressasasflls：Firstly,500gBuxusirphyllaleaveseretakenintrundflask,atthesaetieadded3000l70%ethylalhl,thenheatedbakfl,

8、ttallythreeties,threehurseahtie.Afterextratingthefiltratin,ergedthefiltrate.Finnallythegrssextratserenentratedtnn-alhlflavr.Thegrssextratsereextratedithbenzine,ethylaetate,hlrfr,nralbutylalhlrespetivelyardingtreslverplarityrderfrsalltbig,theneveryextratedliquidasevapratedntheaterbathtgeteahvariuspar

9、tfextratins(afterultipleithdras,btainsstablefingerprintatlas).henexperientbEingarriedn,eahextratinasdisslvedith2%Tain-80distilledater.1.1.2ReagentsXueZhiKangapsule(BEIjingUniversity,BLBiteh,LTD.Bathnuber:20220513);Ttalhlesterlreagentkit(ZheJiangDnguBilgialEngineering,LTD.Bathnuber:2022120220);Trigly

10、eridedeterinatinreagentkit(ZheJiangDnguBilgialEngineering,LTD.Bathnuber:2022120223);therreagents,analytialreagentavailablenarket.1.1.4Instruents722-delUV-visiblespetrphteter(ShanghaiPreisinSientifiInstruent.,LTD);TGL-168high-speeddesktpentrifuges(ShanghaiAntingSientifiInstruentFatry);Digitaltheateda

11、terbathpt(ZhengzhuGreatallSientifiIndustrialTrade.,Ltd).轉(zhuǎn)貼于論文聯(lián)盟.ll.1.2ethds1.2.1Divisinandadinistratinnehundredandthirtyie,aleandfeale,Eight(25.02.0)g,ererandlydividedint13grups,eahith10,naely:ntrlgrup;delgrup;XueZhiKanggrup;aterextrathigh-dse;aterextratl-dse;Petrleuetherextrathigh-dse;Petrleuethere

12、xtratl-dse;Ethylaetateextrathigh-dse;Ethylaetateextratl-dse;hlrfrextrathigh-dse;hlrfrextratl-dse;n-butylalhlextrathigh-dse;n-butylalhlextratl-dse.Sixteenhursafterfasting,theiefgrup(1)and(2)eregivenslvent(distilledaterinluding2%Tain-80,0.2l/10g)bygavage;theiefGrup(3)eregivenXuezhikang(0.24g/kg)byintr

13、agastriadinistratin;Experientalhigh-dsetreatentgrupseregavagerrespndingdrug0.90g/kg;l-dsegruperegavagerrespndingdrug0.45g/kg.Allabvegrupsdeliveryapaitiesere0.2l/10g.Thurslater,exeptblankgrupallthetherieereinjetedintraperitneallyith75perenteggylkeulsin,dse0.1l/10g(75perenteggylkeulsin:eggylkfr1-2dfre

14、sheggsathedithsaline).Sixhurslater,eahgrupasadinisteredbygastriperfusinrrespndingslventrtheediinenere,thedsagedett,tentyhursafterinjetinfegg-ylkeulsin,bldasextratedfrthephthalivenusplexus,thenbldseruasseparated(3000RPperinute,entrifugal15inutes).Finally,seruttalhlesterl(T)andtriglyeridentent(TG)eree

15、asured6,7,DeterinatinTtalhlesterlasdeterinedbyHD-PAP;triglyeridesasdeterinedbyGP-PAPlaperatedardingtspeifiatin.1.2.3StatistialtreatentTheeasureentdataereexpressedaseanstandarddeviatin(s)andanalyzedbyANVAithSPSS10.0sftare.2ResultSeruttalhlesterlandtriglyeridelevelsfthedelgruperehigherthanthsei

16、nthentrlgrup,thediffereneserestatistiallysignifiant(P0.01),hyperlipideiadelsuessed.Inntrastiththedelgrup,thelevelsfseruttalhlesterliniefaterextratsfhigh-andl-dsegrupsandpetrleuetherextratsfhigh-andl-dsegrupsandethylaetateextratsfhigh-andl-dsegrupsereler,thediffereneserestatistiallysignifiant(P0.01).

17、parediththedel,thelevelsftriglyerideiniefethylaetateextratsfhigh-andl-dsegrupserelerthanthatfdelgrups,thediffereneserestatistiallysignifiant(P0.01),buttheaterextratsfhigh-andl-dsegrupsandpetrleuetherextrathigh-andl-dsegruphadnstatistiallydifferene(P0.05).parediththedelgrup,thententsfttalhlesterlandt

18、riglyerideshlrfrextratsfhigh-andl-dsegrupsandn-butylalhlextratsfhigh-andl-dsegrupshadnsignifiantstatistialdifferene(P0.05).Seetable1.Table1TheeffetsfthedifferentextratsfrBuxusirphyllaleafnTandTGinhyperlipideiadelie.略3DisussinHyperlipideiaresultedfrlipidetablisdisrderinthebdyisandiseaseintheelderly,f

19、requently-urringdisease,anditisariskfatrleadingtarterislertiardivasulardisease,seriuslydesharthuanhealth.iththeiprveentflivingstandards,theppulatinfhyperlipideiaisinreasing,thedeandfhuanthyplipideidrugsisngring,hene,thedevelpentfhyplipideiagentshavegdsialandenibenefits9,10.Ansiderableauntfresearhshs

20、thatalkalidsislatedfrdfBuxusirphylla(suhasylvirbuxineD)haveabetterlinialeffetnardivasulardisease.Hever,thestudyntheBuxusirphyllaleafisless,andthestudynitshyplipideiisntrereprtedinliteratures.Inthisstudythesysteslventasusedtgetdifferentextrats.AtthesaetiethEIreffetsniehyperlipideiaerebserved.Theresul

21、tsshthatethylaetateextratsfrBuxusirphyllaleaf(0.45-0.90g/kg)signifiantlyreduelevelftheseruttalhlesterlandtriglyerideinhyperlipideiaie.ItprvidestheexperientaldatafrfurtherstudynhyperlipideiativeingredientsandexplreGuangxisrihplantresures.【參考文獻(xiàn)】1ZhangKaiYuan，uYiYuan.ThereviefthehuangyangninglinialuseJ

22、.priaryjurnalfhineseateriaedia,2000，144：52.2LiuRuYing.TheliniusefthehuangyangningtabletJ.Jurnalflinialinternalediine,1997，142：112.3TaXinYietal.PrtetiveeffetsfVB-DntheerebralisheiainjuryJ.Pharalgyandlinisfhineseateriaedia,2022，19(5):13.4ZhaYngei，LlFaRng，YANGJIANXING，etalStudynthereduingbldlipidandantixiditineffetfphillyrinJ.Natureprdutinresearhanddevelpent2022，172：157.5ZhangRng,FengaLl,uYuPeng,etal.Experientalstudyntheativesitusffetidassiaseedtr