Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEDigoxigeninCat.No.:HY-B1025CASNo.:1672-46-4Synonyms:Lanadigenin分?式:C??H??O?分?量:390.51作?靶點:Others作?通路:Others儲存?式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性數(shù)據(jù)體外實驗DMSO:125mg/mL(320.09mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制備儲備液1mM2.5608mL12.8038mL25.6075mL5mM0.5122mL2.5608mL5.1215mL10mM0.2561mL1.2804mL2.5608mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；?旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲備液的保存?式和期限：-80°C,6months;-20°C,1month。-80°C儲存時，請在6個?內(nèi)使?，-20°C儲存時，請在1個?內(nèi)使?。體內(nèi)實驗請根據(jù)您的實驗動物和給藥?式選擇適當(dāng)?shù)娜芙?案。以下溶解?案都請先按照InVitro?式配制澄的儲備液，再依次添加助溶劑：(為保證實驗結(jié)果的可靠性，澄的儲備液可以根據(jù)儲存條件，適當(dāng)保存；體內(nèi)實驗的?作液，建議您現(xiàn)?現(xiàn)配，當(dāng)天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的?式助溶)1.請依序添加每種溶劑：10%DMSO>>40%PEG300>>5%Tween-80>>45%saline1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemESolubility:≥2.08mg/mL(5.33mM);Clearsolution2.請依序添加每種溶劑：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥2.08mg/mL(5.33mM);Clearsolution3.請依序添加每種溶劑：10%DMSO>>90%cornoilSolubility:≥2.08mg/mL(5.33mM);ClearsolutionBIOLOGICALACTIVITY?物活性Digoxigenin(DIG)?種類醇。由于DIG具有保質(zhì)期長、檢測速度快等優(yōu)點，被?于原位雜交?靈敏度的DIG標(biāo)記核糖探針。體外研究DigoxigeninisapotentactivatorofRORγ-dependenttranscriptionat1μMwithanEC50valueof0.358μM[2].CellViabilityAssay[2]CellLine:HepG2(humanhepatocellularcarcinoma)celllineConcentration:0-10μMIncubationTime:24hResult:IdentifiedtheoptimalconcentrationforDigoxigenin:1μM.CellCytotoxicityAssay[2]CellLine:RORγ-HepG2cellsConcentration:0-2μMIncubationTime:24hResult:Increasedtheluciferaseactivitywithconcentration-dependentincells.REFERENCES[1].Luo,Yuhao,etal.LongnoncodingRNA(lncRNA)EIF3J-DTinduceschemoresistanceofgastriccancerviaautophagyactivation.Autophagyvol.17,12(2021):4083-4101.[2].Kara?,Kaja,etal.Thecardenolidesstrophanthidin,digoxigeninanddihydroouabainactasactivatorsofthehumanRORγ/RORγTreceptors.Toxicologylettersvol.295(2018):314-324.[3].Barratt,KristenS,etal.ProductionofDigoxigenin-LabeledRiboprobesforInSituHybridizationExperiments.Currentprotocolsinmousebiologyvol.10,2(2020):e74.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchu