1、尿液是早期敏感的標志物來源尿液是早期敏感的標志物來源 生物標志物是疾病相關(guān)的變化 尿液積累大量變化，而血液中的變化被盡快清除 所以尿液的變化更早期更敏感2型糖尿病模型動物 ZDF (Zuker diabetic fatty rat)大鼠是公認的先天遺傳性2型糖尿病和肥胖病模型動物。它具有遺傳性胰島素受體缺失的特點。ZDF大鼠發(fā)展為2型糖尿病也常并發(fā)高血脂和高血壓，在生化代謝、組織形態(tài)改變和心血管并發(fā)癥等方面與人類很相似，所以是此動物模型是一種非常理想的研究2型糖尿病及其并發(fā)癥的動物模型。糖尿病模型的尿液早期變化ZL對照大鼠及ZDF模型大鼠空腹血糖及尿糖濃度變化博來霉素誘導(dǎo)的大鼠肺纖維化Day

2、7Day 14Day 21Day 28H&E (A-D) and Massons trichrome staining (E-H) of lungs of BLM rats ondays 7, 14, 21 and 28 days after BLM instillation. Massons trichrome stainingrevealed no obvious fibrosis in the lungs on Day 7, only limited lung interstitialfibrosis on Day 14 and a large number of collagen fi

3、bers on Day 21.肺纖維化模型的尿液早期變化Day 6Vimentin is a tissue fibrosis marker In our previous study, vimentin was up-regulated in the urine of rats with kidneyfibrosis. The expression of vimentin wasalso increased in the urine in ourexperiment.XIRP2 - AngII - Fibrosis Xin actin-binding repeat-containing pro

4、tein2 (XIRP2) is another protein that wasfound to be up-regulated in urine. XIRP2may disturb AngII signaling, which isknown to enhance fibrosis.LRP4-Wnt-Idiopathic pulmonary fibrosis The Wnt signaling pathway is associatedwith idiopathic pulmonary fibrosis, andlow-density lipoprotein receptor-relate

5、dprotein 4 (LRP4) may be involved in thenegative regulation of the Wnt signalingpathway.不同時間點開始的激素治療第8天第15天第22 天未治療都截止在28天尿液有可能是腫瘤早期標志物的來源嗎？Subcutaneous tumor modelTiny tumor mass could be touch in a part of W256 rats beginning from the sixth day腫瘤模型尿蛋白改變Day04691114腫瘤動物模型的尿液早期變化尿差異蛋白 24 differential

6、 proteins in this experimentwere annotated as cancer biomarkers 27 proteins were associated with breastcancer in previous studies 9 of them were annotated as biomarkersof breast cancer腫瘤相關(guān)的差異尿蛋白 B2MG were found to be urine markers ofseveral cancers PDC6I was potential urinary biomarker ofupper gastr

7、ointestinal cancer CO4 in human urine was reported to behelpful in diagnosis of bladder cancer NGAL was a potential biomarker formalignancy KNT1 was validated to be urine marker ofbreast cancerUrimemUrinary Proteins on Membrane尿中microRNA的保存家用尿液保存裝置絨毛漿紙尿褲收集尿液淚液的干燥保存淚液保存的比較不同個體淚液變化大淚液里各個組織富集的蛋白On protein level160140120100806040200淚液不是金礦可能也是銀礦Biobankings future:Preserving easily acquired informativesamples long-term at room temperatureUrimem的價值取決于 生物學(xué)樣本的質(zhì)量（容易被認識） 晨尿，二次尿，24小時尿，隨機尿，中段尿 保存的方式，是否離心，是否有其他前處理， 更取決于樣本相關(guān)信息的質(zhì)量（尿液受各種因素影響的特殊性） 要記錄得盡可能詳細，除了病歷中