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免疫組織化學(xué)

DoublePeroxidase(HRP)ImmunohistochemicalLabelingofTrypsin-SensitiveAntigens(KPL)

Immunohistochemistry(Tynerlab)

Thisisabasicimmunohistochemistryprotocol.Protocolsforimmunohistochemistryusr/localywidely,duetothedifferencesbetweenantigensandtheirrecognitionbyantibody.Forsuccessitiscrucialtotryanumberofdifferenttechniquesandcomparetheresults.

Treptavidin/Beta-GalactosidaseImmunohistochemicalLabelingProcedure(KPL)

ImmunohistochemicalStainingofFloatingThickSections(10-100microns)(KPL)

GeneralProtocolforstainingthicksections

ImmunohistochemicalStainingforFormalin-Fixed,Paraffin-EmbeddedTissues(L

EP)

Thisstainingprocedureisfortissuesfixedin10%neutralbufferedformalinandembeddedinparaffin

ImmunohistochemicalStain-AlkalinePhosphatase(APAAP)Technique(Nottingha

mPathologyLab)

Immunohistochemisty-EnzymaticProtocol(R&D)

Generalprotocolincludingdetailsforsamplepreparation,tissuestaining

andtroubleshooting

Immunohistochemisty-FluorescenceProtocol(R&D)

Generalprotocol

ImmunohistochemistryofMouseFrozenTissues(eBioscienee)

Immunofluorescence-WholeMount一(KarlClark)

Immunohistochemistryallowsvisualizationofantigens(usuallyproteins)

withinanembryo.Typically,aprimaryantibodybindsspecificallytoanantigen(e.g.Luciferase);thenasecondaryantibodyconjugatedtoaflourochromeorenzymeisboundtotheheavychainconstantregionoftheprimaryantibodywithspecificitytothespeciesoforiginoftheprimary(e.g.anti-rabbitconjugatedtoRhodamine

ImmunohistochemistryonWhole-MountEmbryos(VesicleTrafficking)

WholemountStainingofEmbryos(XMMR)

Beta-galStaining-WholeMount(KarlClark)

Beta-galstainingallowsidentificationofembryonictissues/cellsexpressinglacZmarkerproteinbydevelopmentofpigmented(blue)productinthepresenceoflacZenzymaticactivity.

X—gal&APStainingProtocol(CepkoLab)

Verydetailedprotocolwithbackgroundinformationandseveralapplications.

ReducingBackgroundStaininginImmunohistochemistry(KPL)

HistochemicalVisualizationofMycoplasmaparatuberculosis(KPL)

SolutionsandCalculationsforImmunohistochemistry(KPL)

Asemi-permenantmountingmediumforimmunofluorescencemicroscopy(Hous

eEarInstitute)

ArecipeforMoviol,

aninexpensivemountingmediumthatiseasytoprepare.

MicrowaveImmunohistochemistry(EnergyBeamSciences,Inc.)

Introductiontomicrowaveimmunohistochemistrytechniqueanditsappli

caitonpr

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