豬場(chǎng)引種生物安全關(guān)鍵點(diǎn)Thekey

pointsofbiosecurityto

inducedpigsPIC?生物安全盾（PIC?Bioshields）活豬和精液?jiǎn)?dòng)禁售豬場(chǎng)設(shè)施糞污處理人員食物，蔬菜，廚房等控制Food,Vegetable,Kitchen設(shè)備和物資病死豬處理management+.

主動(dòng)被動(dòng)監(jiān)測(cè)和禁售-常規(guī)監(jiān)測(cè)飼料運(yùn)輸引自PIC亞太健康保2

障總監(jiān)姚建聰-售前檢測(cè)主要內(nèi)容

Maincontents?

種源場(chǎng)的健康是至關(guān)重要的Thehealthstatus

ofsouringfarm

iscritical.?

種豬車輛的準(zhǔn)備、裝豬和運(yùn)輸Thepreparation

oftruck,loadingandtransportation.?

隔離舍的選擇、設(shè)計(jì)與卸豬Isolationselection,designandunloading.一、種源豬場(chǎng)的健康Thehealthstatus

ofsouringfarm?

對(duì)種豬健康要求及如何驗(yàn)證引種來(lái)源豬只健康Therequirement

ofhealthstatus

andhowto

identifyhealthstatus.?

種源豬場(chǎng)的市場(chǎng)口碑與持續(xù)供應(yīng)能力Thepublicpraise

andcontinuoussupplyability.?

與種源豬場(chǎng)的獸醫(yī)溝通健康管理程序和采樣檢測(cè)Communicationswiththevet

ofscouringfarm

abouthealthassuranceprogram

andsamplegathering.?

種豬到場(chǎng)后的健康監(jiān)測(cè)Thehealthmonitorprogram

after

arrival.1、對(duì)種豬健康要求及如何驗(yàn)證種源場(chǎng)豬只健康Therequirement

ofhealthstatus

andhow

to

identifyhealth?

參考關(guān)注的疾病種類Thediseasespeciesyou

may

focus

on.病毒?。悍侵挢i瘟、豬繁殖呼吸綜合征（藍(lán)耳?。?、豬瘟、豬偽狂犬、豬流行性腹瀉（PED）、輪狀病毒和豬口蹄疫等Virus:ASF,

PRRS,

CSF,

PR,PED,

RV

andFMD.etc細(xì)菌?。贺i支原體、傳染性胸膜肺炎、豬萎縮性鼻炎和豬丹毒等Bacteria:MH,APP,

ARandSE(SwineErysipelas).etc1、對(duì)種豬健康要求及如何驗(yàn)證種源場(chǎng)豬只健康Therequirement

ofhealthstatus

andhow

to

identifyhealth?

檢測(cè)方法：聚合酶鏈?zhǔn)椒磻?yīng)（PCR）、酶聯(lián)免疫吸附反應(yīng)（ELISA）、病理切片、免疫熒光、免疫組化和病毒中和試驗(yàn)等Test

methods:PCR,ELISA,PathologicalSection,IFA,

IHandVN.etc?

檢測(cè)結(jié)果的可解釋性，如藍(lán)耳陽(yáng)性穩(wěn)定場(chǎng)檢測(cè)結(jié)果的挑戰(zhàn)Howto

interpret

thetest

result,for

instance,

thetest

result

from

PRRSstable

farm.2、種源豬場(chǎng)的市場(chǎng)口碑與持續(xù)供應(yīng)能力Thepublicpraise

andsupplyabilityofsourcing

farm?

來(lái)自種豬場(chǎng)過(guò)往引種客戶的反饋Thefeedback

from

theclientswhohadinducedpigsfrom

scouringfarm.?

種豬場(chǎng)的數(shù)量和全國(guó)的布局Thenumberofbreeding

farms

andmultiplierherds

distributioninChina.?

是否有持續(xù)供應(yīng)種豬的能力Theabilityto

supplybreeders

constantly.3、與種源豬場(chǎng)獸醫(yī)溝通健康管理程序和采樣檢測(cè)Communicationswithvet

ofsourcing

farm

aboutthehealthmanagementprogram

andsampletest?

種源豬場(chǎng)的健康保障程序

Thehealthassurance

program.?

種源場(chǎng)獸醫(yī)團(tuán)隊(duì)的專業(yè)性

Profession

ofvet

team.?

過(guò)往最近三個(gè)月檢測(cè)報(bào)告

Thetest

reportsinthepast

3months.?

根據(jù)關(guān)注疾病制定采樣計(jì)劃和檢測(cè)結(jié)果的解讀According

to

theconcerneddiseasesto

make

upsampleplanandresult

explanation.示例：PIC中國(guó)豬群監(jiān)測(cè)計(jì)劃Example:

Monitorprogram

ofPICChinaherds種豬群健康監(jiān)測(cè)流程HealthmonitorprogramSTEP1第一步HoldalldeliveriesfromFarm.

Closefor

breedersales.Checkfor

clinicalsignsat

farm*

暫停所有運(yùn)輸，與種豬銷售，檢測(cè)豬群臨床癥狀SubmissionofSamplestoMainLaboratory提交樣品Positiveresults陽(yáng)性結(jié)果STEP2第二步Request

retest

at

LabAllClear結(jié)果沒(méi)問(wèn)題ClinicalsignsObservedat

Farm.ClosureSustained.ControlMeasuresImplemented.

如有臨床癥狀，則封場(chǎng)，執(zhí)行控制策略要求實(shí)驗(yàn)室重新檢測(cè)Positiveresults/Noclinicalsigns陽(yáng)性結(jié)果

無(wú)臨床癥狀Farm

isopenforBreederSales農(nóng)場(chǎng)開(kāi)始銷售/STEP3第三步

ResubmitretainedSamplesat

farm

for

retest

重新提交備用樣品進(jìn)行檢測(cè)*Clinicalsignsat

farm

willeithersupportorquestionsresults.臨床癥狀的觀察支持或者質(zhì)疑檢測(cè)結(jié)果Positiveresults/Noclinicalsigns陽(yáng)性結(jié)果/無(wú)臨床癥狀STEP4第四步Collectnewsamplesat

farm

重新采樣OrganizedbyDrAngelC.ManabatS/P值分組藍(lán)耳支原體?

血清學(xué)檢測(cè)結(jié)果來(lái)自于美國(guó)和加拿大兩個(gè)持續(xù)監(jiān)控藍(lán)耳和支原體的核心群。通過(guò)對(duì)研究期間獲得的S/P值及陰性，疑似和陽(yáng)性結(jié)果所占比例進(jìn)行總結(jié)分析。此外，通過(guò)結(jié)合PCR檢測(cè)結(jié)果數(shù)據(jù)，證實(shí)豬場(chǎng)維持了上述藍(lán)耳病和支原體的陰性狀態(tài)。?

Serologictest

results

from

two

nucleusherds

locatedinUSandCanadathat

are

constantly

monitored

forPRRS

andMHwere

usedfor

thisstudy.

Data

wassummarizedandanalyzed

basedons/pratios

andproportionofnegative,

suspectandpositiveresultsthroughthestudyperiod.Inaddition,data

from

PCRtest

results

from

thesamefarm

was

usedto

confirmthat

theherd

maintaineditsna?ve

status

for

thetwomentionedpathogens.假陽(yáng)性率

FalsePositiverate0.31%2.1%4、種豬到場(chǎng)后的健康監(jiān)測(cè)

Healthmonitorafter

pigarrival?

種豬到達(dá)后的采樣檢測(cè)，以評(píng)估運(yùn)輸種豬與種源場(chǎng)的健康的一致性，需要雙方認(rèn)可的第三方實(shí)驗(yàn)室Thetest

after

pigarrival

to

evaluate

thehealthstatus

compared

withsourcing

farm,

thethird-partytestinglabapprovedbybothparties.?

豬只臨床癥狀的觀察和應(yīng)急采樣檢測(cè)Monitortheclinicalsignsandemergencysamplegathering.?

隔離解除入群前的健康檢測(cè)二、種豬車輛的準(zhǔn)備、裝豬和運(yùn)輸Thepreparation

oftrucks,loadingandtransportation?

種豬車輛的選擇與洗消Theselection,wash

anddisinfectionoftrucks.?

種豬車輛在種源場(chǎng)裝豬Theloadingprocess

oftruckinsourcing

farm.?

車輛運(yùn)輸途中生物安全Thebiosecuritymanagement

intransportation.1、種豬車輛的選擇與洗消Theselection,wash

anddisinfection

oftrucks?

不同類型車輛都可以做為種豬運(yùn)輸車Different

truckscan

beusedfor

deliverbreeders.?

車廂設(shè)計(jì)是決定清洗質(zhì)量的關(guān)鍵因素Thedesignoftruckcarriage

isveryimportant

to

thewash

quality.?

車輛清洗干凈是成功運(yùn)輸?shù)幕緱l件Cleantruckisthebasicconditionsto

successfuldelivery.?

高質(zhì)量消毒為成功的運(yùn)輸而保駕護(hù)航Highqualitydisinfectioniscriticalfor

delivery.傳統(tǒng)的高欄車Traditional

truck半密閉運(yùn)輸車Semienclosedtruck不同車輛連接處設(shè)計(jì)Thejoint

designofdifferent

trucks√×空氣過(guò)濾運(yùn)輸車Truck

withairfilter

equipment百葉窗Louver進(jìn)風(fēng)口Airintake空氣過(guò)濾室Airfilterchamber空氣過(guò)濾車頂板膠條損壞封閉不嚴(yán)Theceilingoffiltertruckisnotclosedcompletely.頂板內(nèi)表面Theinsidesurface

ofceiling.頂Theoutsidesurface

ofceiling車輛洗消是關(guān)鍵Thewash

anddisinfectionoftruckiske

y.車輛檢查Inspectionto

truckcarriage.未清洗干凈的空氣過(guò)濾車Airfilter

truckisnotclean.車底盤清洗干凈容易嗎？Isiteasy

to

cleanthebottom

oftruck?洗車點(diǎn)

Truck

wash

site25公分高*60公分寬*3.5米長(zhǎng)前面4個(gè)輪上坡，瀝水30分鐘熱氣筒暖氣片保持室溫

Heater

radiatorkeep

theroom

temperature瀝水架Inflator

channelDraining

board消毒和烘干之前車輛要瀝干Thetruckshouldbedrybefore

disinfectionanddrying.烘干結(jié)束之后，車輛上還有積水Residuarywater

inthetruckafter

drying.左圖試驗(yàn)數(shù)據(jù)表明如果車輛未充分瀝水，即使有尾部風(fēng)機(jī)和長(zhǎng)達(dá)0分鐘烘干時(shí)長(zhǎng)，車廂內(nèi)某些位也無(wú)法達(dá)到目標(biāo)溫度。hetruckisnotdrainedmpletely,

thetruckcan

notbeto

target

temperature

even

ifites160minutesto

dry.引自PIC健康保障經(jīng)理夏天Cite

from

TianXiaPICHA不要在商業(yè)卡車清洗點(diǎn)清洗種豬車Donotwash

thededicatedtruckincommercial

truckwash

site2、種豬車輛在種源場(chǎng)裝豬Theloadingprocess

oftruckinsourcing

farm?

車輛停靠點(diǎn)到種源場(chǎng)路線確定Theroute

from

truckparkingsite

to

scouringfarm.?

運(yùn)輸人員衣服、鞋和食物準(zhǔn)備Thepreparation

ofclothes,shoesandfood

for

drivers.?

運(yùn)輸前車況和補(bǔ)給的再次確認(rèn)Reconfirm

thetruckconditionandoilmass.?

種源場(chǎng)裝豬生物安全的全過(guò)程Theloadingprocessing

inscouringfarm.車輛停靠點(diǎn)到種源場(chǎng)路線確定Theroute

from

truckparkingsite

to

scouringfarm?

車輛?？奎c(diǎn)選擇合適地點(diǎn)，到種源場(chǎng)的路線盡量避開(kāi)村莊、農(nóng)貿(mào)市場(chǎng)和養(yǎng)豬密集區(qū)，道路最好是硬化的道路Theroute

from

truckparkingsite

to

scouringfarm

shouldapartfrom

villages,wet

market

andhigh-densitypigproduction,theroad

shouldbeconcrete.?

計(jì)算路途時(shí)間，根據(jù)卸豬時(shí)間確定車輛出發(fā)的時(shí)間Calculate

thetimefrom

parkingsite

to

scouringfarm

to

scheduledeparttime.?

如果種源場(chǎng)附近有專屬洗消點(diǎn)，可以提前一天抵達(dá)Ifthere

istruckwash

facilitybesidesowfarm,

thetruckscan

bethere

onedayinadvance.種源場(chǎng)裝豬生物安全的全過(guò)程Theloadingprocess

oftruckinsourcing

farm?

對(duì)裝豬設(shè)施洗消和做好人員安排Wash

anddisinfect

theloadingfacility,

prepare

thelabors

for

loading.?

門衛(wèi)對(duì)裝豬車輛按清單進(jìn)行檢查Thedoorguard

checkthedriverandtruckbefore

loading.?

車輛外表進(jìn)行洗消和靜置后裝豬Wash

anddisinfect

thesurface

ofthetruck,downtime

before

loading.?

種豬裝載過(guò)程仍然是三段式裝豬Theloadingprocess

isdividedthree

sectionsto

control

cross

contamination.種豬車在種源場(chǎng)裝豬Loadingpigsinsourcing

farm卡車裝豬人員Staff

inthetruck（紅色區(qū)域

Red

area）豬場(chǎng)內(nèi)生產(chǎn)區(qū)人員

（綠色區(qū)域）Staff

inproductionarea

(Greenarea)生產(chǎn)區(qū)外人員進(jìn)入裝豬設(shè)施工作示例Example

thestaffoutofproductionto

enter

intoloadingfacility3、車輛運(yùn)輸途中生物安全Thebiosecuritymanagementintransportation?

提前了解天氣情況，盡量減少極端天氣的運(yùn)輸Pay

more

attention

to

theweather

to

reduce

thetransportation

inbadwhether.?

運(yùn)輸路線盡量選擇高速，原則是避開(kāi)人員、村莊和集市等Doyour

best

to

choosehighway

to

avoid

people,villagesandwet

market

.etc?

盡量減少停車，除非是加油、加水和上衛(wèi)生間等Except

oilandwater

replenishment,bathroom,

donotparkthetruck.3、車輛運(yùn)輸途中生物安全Thebiosecuritymanagementintransportation?

建立微信群和實(shí)時(shí)地圖群組，監(jiān)控整個(gè)運(yùn)輸過(guò)程Set

upwechat

andreal

timemapgroups

to

monitorthetransportation.?

跨省需下高速檢驗(yàn)健康碼、動(dòng)檢蓋章、指定通道進(jìn)入Pay

more

attention

to

healthcode

check,officialstamp

anddedicated

access.?

提前與接收種豬方溝通，前來(lái)接應(yīng)，代為協(xié)調(diào)等Contact

withcustomers

to

coordinate

thetruckat

highway

station.?

接收種豬方政府對(duì)于新冠疫情防控要求，避免司機(jī)被管控的風(fēng)險(xiǎn)Work

withcustomers

to

knowtheCOVID

prevention

requirement

to

avoidthedrivers

notto

beallowedto

leave

thehighway.三、隔離舍的選擇、設(shè)計(jì)與卸豬Isolationselection,designandunloading?

合格隔離舍的基本要求Thebasicrequirement

ofqualifiedisolation.?

隔離設(shè)施內(nèi)的硬件要求Thefacilityrequirement

intheisolation.?

隔離舍卸載種豬的流程Theunloadingprocessing

intheisolation.1、合格隔離舍的基本要求Thebasicrequirement

ofqualifiedisolation?

隔離舍距離豬場(chǎng)豬群不得低于300米，并有物理隔離Thequalifiedisolationshouldbe300meters

apartfrom

sowherd.?

隔離舍需搭建物理隔檔，理想情況下，有天然屏障Thefence

isneededaround

isolation.Ideally,

there

isnatural

barrier.?

需要具備基本的生物安全設(shè)施，如洗澡間、物資消毒間、人員居住宿舍和散裝料塔等Thebasicbiosecurityshouldbeprepared,

for

instance,

showerin/outfacility,

disinfectionroom,

dormitoryandfeed

bins.etc6000頭母豬場(chǎng)布局

Thelayout

ofsowfarm

(6000sows)干燥房DryingroomwithTADD中轉(zhuǎn)料塔Transfer

feed

bins門衛(wèi)Doorguard臺(tái)Loadingfacility內(nèi)部洗車點(diǎn)Internaltruckwashsit區(qū)Livingandofficeareas圖片來(lái)源云南神農(nóng)Source

fromYunnan食堂和隔離區(qū)Canteenanddowntimearea配懷舍BreedingGestationbarn舍

BoarstudShennong后備培育舍

GDU洗澡間和消毒間ShowerroomsandDisinfectionroomPrimaryCompostPile產(chǎn)房Farrowingroom生活區(qū)Livingarea1000頭母豬場(chǎng)，一點(diǎn)式配懷舍B公豬舍G1000sow

farm，onesite育肥舍FinisherbarnscLagoon出豬臺(tái)Loadingfacility392、隔離設(shè)施內(nèi)的硬件要求Thefacility

requirement

intheisolation?

隔離舍有降溫和加熱設(shè)備There

shouldbeheater

andcoolerfacility.?

隔離舍空間能滿足豬場(chǎng)引種數(shù)量要求，大欄內(nèi)料槽的線性采食空間和飲水器數(shù)量應(yīng)充足Enoughpen/feederspace,nippledrinkers

for

pigs.?

在需要