1、Microbiology and plant interactions,resistence on heavy-metal,Heavy metals being transferred to humans via the food chain would finally threaten human beings health.,Bioremediation on heavy metals,Plant rhizosphere bacteria Plant endophytic bacteria,Grow in plant root system . With bacteria, actinom

2、ycetes, fungi, algae, and the protozoa. Alternate relationship between them and plant. Plant roots interaction and promote each other.,Bacteria,Widely distributed, many varieties, fast metabolism and reproduction. Chemical composition of bacterial cell walls contain many heavy metal ions adsorption

3、groups, such as carboxyl, amino, hydroxyl .,Cyanobacteria,Fungi,Small mold, single-celled yeast and fruiting body of large-scale fungus. Their cell walls contain consists largely of chitin and glucan. Fungi have potential for heavy metal adsorption ability.,Saccharomyces Cerevisiae,White-rot fungi a

4、dsorbe Pb2 + ,the highest removal rate can reach 95%.Black rhizopus adsorbe Ag +, Pb2, Li + and Cd2 +, Zn2 + and Ni2 +, Cu2 +, such as heavy metal ions.,Alage,Wide distribution, a good biological adsorbent. The walls of algae has a larger surface area. Cell walls have a certain charge and viscous, t

5、hese characteristics make the metal ion has the ability.,Scenedesmus Obliquus,Has a larger adsorption capacity to UO22 +. The UO22 + concentration in the water from 5.0 mg/L fell to 0.05 mg/L.,A kind of parasitic bacteria in plant body, all plants have endophytes. Endophytic bacteria (PGPB) is a kin

6、d of parasitic life, promote the growth of the host . Can enhance plant resistance to pathogens, drought, heavy metal.,Endophytic bacteria,From Elsholtzia splendens and duck plantar grass (Commelina communis) .,Firmicutes ,Actinobacteria, Proteobacteria. These bacteria strains in copper resistance o

7、f main groups .,The Cr6+ resistant plant growth promoting bacteria (PGPB), Pseudomonas sp. PsA4 and Bacillus sp. Ba32 were isolated from heavy metal contaminated soils and their plant growth promoting activity on the Indian mustard.,Release of chelating agent and iron carrier. Acidification of the s

8、oil environment, Change the REDOX potential. Reduce the absorption. Increase the discharge Oxidation reduction,Mode of resistance to heavy- metal,Mechanisms of resistence to heavy -metal,Reduce the absorption the heavy metal content in the microbial cells at a low level is maintained without causing

9、 toxic effects on cells. As a type of staph resistance of Cd2 + is the case.,Increase the discharge some microorganisms may be proactive manner by heavy metal ions within the cell is discharged outside the cell, thus maintaining low levels of the cell. As one kind of resistance to Bacillus copper.,O

10、xidation reduction many microbial oxidation or by reduction (in most cases) from the high toxicity of the heavy metal transformed into less toxic valence valence state, thereby lifting the heavy metal toxicity. For example, the resistance of mercury that is the case.,Bonded to the outer cells Reduci

11、ng the concentration of toxic heavy metals in the environment, the purpose of detoxification. Escherichia coli copper resistance effect as that is so, it can be secreted proteins capable of binding to copper ions, thereby reducing the effective concentration of copper ions.,Adsorption Many microbial

12、 cell surface has a special structure, can absorb heavy metal ions and thus to reduce the concentration of heavy metal ions in solution.,Current research progress,Mustard (Brassica juncea) and Cr -resistant strains .,Mustard,Increase biomess,resault,Cr -resistant strains Pseudomonas sp. PsA4 and Bac

13、illus sp. Ba32 applied to mustard (Brassica juncea) . The results showed that vaccinated PsA4 mustard or Ba32 have increased in 95.3 mg / g and 198.3 mg / g Cr (6 +) biomass in the soil.,Castor (Ricinus communis) and nickel, zinc resistance strains.,isolate,electe,inoculate,increase,isolated from th

14、e rhizosphere soil microbes , after nickel, zinc resistance screening . elected the heavy metal resistant strains Pseudomonas sp. And Pseudomonas jessenii. inoculated them to castor (Ricinus communis), they found a significant increase castor biomass , and increased the accumulation of Zn, Ni, Cu s .,Prospects,Heavy metal contamination of soil growing problem in many options in phytoremediation has its unique advantages, the focus of the problem is how to increase the resistance of plants to heavy meta