1、五、軟件的設(shè)詈和仗器的連接光譜測定2.12.22.32.4參數(shù)設(shè)定數(shù)據(jù)采集數(shù)據(jù)的保存和通道的刪除結(jié)果處理2.4.1養(yǎng)2.4.2一Fluorometer-On:：instrument：RF-530ISeraNumber：AOI9201536SAROMVerson：2.1ExcitationSitmissionSitEmissionMonochromatorMonochromatorOEEPROM|ExcitationBaseline #初始化班行一系列的檢杳和初置,in切順利通過,對話框中各頊目粥風作如上右圖設(shè)宗就可以開始測定。光譜測定1.:,AcquireMode、Spectrum”Confi

2、gure、Parameters：彈川光譜參數(shù)対話框 # #設(shè)置要測量的光譜類型以及合適的激發(fā)光發(fā)射光的波長或范用；顯不范圉打描速度采樣間隔 若樣品激發(fā)光譜的發(fā)射波快或發(fā)射光譜的激笈波長未知剛在上述對話框中設(shè)詈合適的激發(fā)笈射狹縫雷度靈敏度（控制菠來強度不會過大）,放置樣品在光度計按鍵中點擊込!幺1在彈I1I-I.：I-：-I.-.I.I.-|丨“Search”等待段時間*由儀器給出最優(yōu)波長。I:l1.“Save：放普樣品, # #2.3川；：|.廣File：“ChannelSaveChannel：門二.丨.IOK；-.,：!J廠_rEraseChannelP0SAMPLER廠生廠1廠E廠2廠衛(wèi)廠

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8、icarafterSaw再UnknownFileName：unknownComment：OKStatusCancelIFileChannelEraseChannel：-I：-丨訂Standard-Unknown.i.OK四、動力學；.|LAcquireModeTimeCourse宀Configure、Parameters,在彈出的參數(shù)對話框中選擇激發(fā)發(fā)射光波長；激發(fā)笈射狹縫嵌度，靈敏嵐反應時訕強席范田以：I.-Autv-ManualAtuo,:-.h-.“】I1?1.丄.-.I.:JManual.：-，:.,-.T-丨：12 42mi.iD.DDDLD.OOQn.nnaTm0|放跖汀i溶劑,，放入樣骷，點“一s口RF-E3：MPIDNEK3500EM:397ii115SF-srFW;一左12 #12 #43-：.|-.-.；.:：.I12.3ManipulateDataPrint：Act.Calc：:廠；丿R