1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEBOC-D-FMKCat. No.: HY-13229CAS No.: 634911-80-1分式: CHFNO分量: 263.26作靶點(diǎn): Caspase; Apoptosis作通路: Apoptosis儲存式: Pure form -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實(shí)驗(yàn) DMSO : 125 mg/mL (474.82 mM)* means solubl

2、e, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制備儲備液1 mM 3.7985 mL 18.9926 mL 37.9853 mL5 mM 0.7597 mL 3.7985 mL 7.5971 mL10 mM 0.3799 mL 1.8993 mL 3.7985 mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液，并請注意儲備液的保存式和期限。BIOLOGICAL ACTIVITY物活性 Boc-D-FMK是可滲透細(xì)胞，不可逆的，泛半胱天冬酶抑制劑。抑制TNF-誘導(dǎo)的細(xì)胞凋亡的IC50值為39 M。

3、IC50 & Target Caspase體外研究Apoptosis is a pathway of cell death orchestrated by a family of proteases called caspases. Boc-D-fmkinhibits TNF-stimulated reactive oxygen species (ROS) generation. Boc-D-FMK inhibits apoptosis1/2 Master of Small Molecules 您邊的抑制劑師www.MedChemEstimulated by TNF- with an IC50

4、 of 39 M 1. BocD-fmk at 50 M prevents genistein-induced apoptosis ofp815 cells. Confocal microscopy shows that the release of mitochondrial apoptotic factors is inhibited byBocD-fmk 2.體內(nèi)研究 Boc-D-FMK-fmk effectively attenuates the hepatocyte apoptosis in bile duct-ligated rats and may improve thesurv

5、ival rates after endotoxin challenge 3. A single injection of Boc-D-FMK results in longterm protection ofMNs against root avulsion-induced death for more than 8 weeks and the Boc-D-FMK-treated MNs are able toregenerate their axons into an implanted PN graft and reinnervate the target muscle 4.PROTOC

6、OLAnimal Rats: Boc-D-FMK is dissolved in DMSO. Male Sprague-Dawley rats group 1 (OBBOC-D) undergo commonAdministration 3 bile duct ligation and simultaneously treatment with Boc-D-FMK. The first dose of Boc-D-FMK (1.5 mg/kg) isinjected into the inferior vena cava immediately after bile duct ligation

7、. Subsequent doses of Boc-DFMK (1.5mg/kg twice daily) are given intraperitoneally on the first and second postoperative days. The last dose (1.5mg/kg) is given on the morning of the third postoperative day 3.MCE has not independently confirmed the accuracy of these methods. They are for reference on

8、ly.戶使本產(chǎn)品發(fā)表的科研獻(xiàn) Anal Chem. 2017 Sep 19;89(18):9788-9796. Sci Rep. 2017 Jun 7;7(1):2929. Exp Cell Res. 2018 Sep 1;370(1):103-115.See more customer validations on HYPERLINK / www.MedChemEREFERENCES1. Cowburn AS, et al. z-VAD-fmk augmentation of TNF alpha-stimulated neutrophil apoptosis is compound spec

9、ific and does not involvethe generation of reactive oxygen species.2. Yee SB, et al. zVAD-fmk, unlike BocD-fmk, does not inhibit caspase-6 acting on 14-3-3/Bad pathway in apoptosis of p815 mastocytomacells. Exp Mol Med. 2006 Dec 31;38(6):634-42.3. Sheen-Chen SM, et al. Effect of Boc-D-Fmk on hepatoc

10、yte apoptosis after bile duct ligation in rat and survival rate after endotoxinchallenge. J Gastroenterol Hepatol. 2008 Aug;23(8 Pt 1):1276-9.4. Chan YM, et al. Inhibition of caspases promotes long-term survival and reinnervation by axotomized spinal motoneurons of denervatedmuscle in newborn rats. Exp Neurol. 2003 Jun;181(2):190-203.McePdfHeightCaution: Product has not been fully va