1、 polymerase chain reaction (PCR)Definition of PCRThe basic principle of PCRPCR Frequently Asked QuestionsApplication of PCRDefinition of PCRPCR(polymerase chain reaction),is under the catalytic of DNA polymerase,to mother chain DNA as a template, with specific primers for the the starting point of e

2、xtension, through the degeneration, annealing, extending steps, with the parent chain template DNA replicate the complementary DNA child chain in vitro .It is one of the amplification technique of DNA synthesis in vitro, can quickly specific amplificate any purpose DNA in vitro.The basic principle o

3、f PCR1.PCR reaction components (1) template DNA (2) Primer (3) four kinds of deoxyribonucleotides (4) DNA polymerase (5) Reaction Buffer, Mg2+2. PCR reactions basic steps(1) Denaturation , The hydrogen bonding between the template double chain DNA ruptured by heating, and double chain apart into a s

4、ingle chain.(2) Annealling , As the temperature falls, the primer and complementary area of template DNA combined into hybrid molecules.GGATCTAGCGTATGCTTGAAACCTAGATCGCATACGAACT T TGAACTTTGGATCTA55353353primer1primer2Template DNAPicture1, PCR primers combined with template B. The specific product and

5、 electrophoresis coating shaped1. The concentration of Mg 2+2. Adjust the dosage of the primer, templates, and polymerase3. Decrease the cycle number4. Increasing the annealing temperature, annealing or extension of timeApplication of PCR1. The gene diagnosis of genetic disease2. The diagnosis of infectious diseases (hepatitis virus)3. The cancer gene detection4. The application of forensic science (paternity test)5.