Fluorescentprobes熒光探針廈門大學(xué)化學(xué)系郭祥群郭祥群博士,博士生導(dǎo)師◆全國高校化學(xué)教育研究中心第四屆學(xué)術(shù)委員會副主任,教育部高等學(xué)校本科教學(xué)工作水平評估專家,中國化學(xué)會理事◆先后獲得國家級獎2項,省部級獎4項,發(fā)表科研與教學(xué)研究論文100余篇。◆研究興趣:熒光光譜分析的原理與技術(shù),在研項目涉及生物活性自由基的熒光表征,海洋污染生物監(jiān)測技術(shù)研究,食品安全等?！袈?lián)系方式(L,2187288(0),2182355(H)FluorescentProbes◆Introduction◆Fluorescentsensor熒光傳感◆Fluorescenttracer熒光示蹤◆FRET共振能量轉(zhuǎn)移熒光◆Application1.Radicalprobes5.Proteinprobes2.lonprobes6.DNAprobes3.NIRFFluorescenceimage7.Membraneprobes4.DNAsequence8.pHprobescanRyoFluorescenceresearch◆Keyword:FluorescenPeriodJan/2005-Jun/2005◆Database:美國化學(xué)會(ACcS)數(shù)據(jù)庫3566篇WPS電子期刊全文數(shù)據(jù)庫sD0S全文數(shù)據(jù)庫(上交鏡像1890篇篇英國皇家化學(xué)會(RS0)數(shù)據(jù)庫120篇研究與應(yīng)用領(lǐng)域涉及:生命環(huán)境材料等講座要求了解:熒光探針表征應(yīng)用研究意義MolecularProbeMolecularreporter象鍵ig.1.Fluorescentproberepresentsamolecularreporterinthebiologicalsample.FromJFluorescence3MolecularProbesxpLorer文件()就〔E)查收或A工具(幫助TDrobeSp33.D600Probes②轉(zhuǎn)到錢接“快譯)國回面回國圖圈上網(wǎng)手國·色BioassaysProbes/ewyaCellBiologybMoroNows.MicrobiologywebHighlighTsCatalogcfGenamicsandtMicrosTheLeaderinFlorescenceTechnologyBicerobes44GenomIcsandSYERSafeDNAgelstain-TheMolecularBiologyProteInBiochemistRecentAdditonstoCurproductasecondaryDetectionwSubscribetoprobesonlineIccardJablonskdlagramVibrationalrelax.VR.10-1210-4sInternalconversion.c,10-110-13sLUMOIntersystemcrossing,ISC,10-210-5sAbsorption11sfuorescenceAhvE10-8sVAPhosphorescenceISC1031095NhHOMOCharacteristicsofFluorophoreExcitationwavelength激發(fā)波長axEmissionwavelength發(fā)射波長λmExtinction(absorption)coefficient吸光系數(shù)StokesshiftFluorescencelifetime熒光壽命τFluorescencequantumyield熒光量子產(chǎn)率φFluorescenceAnisotropy各項異性rFluorescenc