15CodeofpracticeforsalttoleranceidentificationofcropvarietiesI水利工程有限公司、呼和浩特農(nóng)牧技術(shù)推廣中心、鄂爾多斯市萬(wàn)通農(nóng)牧業(yè)科技有趙彬、胡文明、朱暉、楊芳、劉先芬、傅莉輝、杜景新、邢1農(nóng)作物品種耐鹽性鑒定技術(shù)規(guī)程耐鹽堿能力saline-alkalinetolerance作物在鹽堿脅迫下，其生長(zhǎng)發(fā)育和產(chǎn)量形成對(duì)鹽堿脅迫的反鹽害指數(shù)saltinjuryindexofseedlings4.1.1種子準(zhǔn)備4.1.2種子處理24.1.3脅迫溶液制備4.1.4脅迫處理4.1.5性狀調(diào)查將培養(yǎng)皿放入光照培養(yǎng)箱中，經(jīng)常檢查溫度℃dd4847458444.1.6萌發(fā)期耐鹽堿性4.1.6.1發(fā)芽勢(shì)脅迫率R1──發(fā)芽勢(shì)脅迫率；1──對(duì)照發(fā)芽勢(shì)，初始計(jì)數(shù)天數(shù)統(tǒng)計(jì)種子發(fā)芽數(shù)；1──處理發(fā)芽勢(shì)，初始計(jì)數(shù)天數(shù)統(tǒng)計(jì)種子發(fā)芽數(shù)。4.1.6.2發(fā)芽率脅迫率R2──發(fā)芽率脅迫率；2──對(duì)照發(fā)芽率，末次計(jì)數(shù)天數(shù)統(tǒng)計(jì)種子發(fā)芽數(shù)；2──處理發(fā)芽率，末次計(jì)數(shù)天數(shù)統(tǒng)計(jì)種子發(fā)芽數(shù)。4.1.6.3根長(zhǎng)脅迫率3R3──根長(zhǎng)脅迫率；3──對(duì)照根長(zhǎng)，末次計(jì)數(shù)天數(shù)時(shí)測(cè)定；3──處理根長(zhǎng)，末次計(jì)數(shù)天數(shù)時(shí)測(cè)定。4.1.6.4鹽堿脅迫率R──鹽堿脅迫率。4.2.1種子要求4.2.2種子處理4.2.3脅迫溶液制備4.2.4脅迫處理4.2.5性狀調(diào)查01234544.2.6抗逆指標(biāo)測(cè)定含量、游離脯氨酸含量、超氧化物歧化酶活性等抗性指標(biāo)進(jìn)行耐鹽堿性評(píng)5.2.1隸屬度確定R(Xi)=·······························································(Xi)=1?···············································i──各指標(biāo)測(cè)定值；min──各測(cè)定指標(biāo)的最小值；max──各測(cè)定指標(biāo)的最大值。5.2.2權(quán)重確定（j=1,2,…,k）···········W（j）──權(quán)重；P（j）──經(jīng)主成分分析所得到各材料第j個(gè)綜合指標(biāo)的方差貢獻(xiàn)率；k──