1、Muscle Tissue 肌組織,Ying Ding Email:,The content,VCD: The function of skeletal muscle Observation: Skeletal muscle 骨骼肌 HE, NO.21 special stain shown LCT in yellow color 熒光桃紅 NO.22 (2) Cardiac muscle 心肌 HE, NO.19 special stain shown intercalated disc 鐵蘇木素染色看閏盤 NO.20 Smooth muscle 平滑肌 HE, NO.23 Homework

2、: Draw the longitudinal and cross section of skeletal muscle fiber in high power objective lens 40, and describe its construction features. 作業(yè)：在高倍鏡下描繪骨骼肌纖維的縱、橫切面，并描述其結(jié)構(gòu)特征。, 肌組織的分類、分布：,分類,分布,光鏡,骨骼肌,心 肌,平滑肌,軀干、四肢,心 臟,內(nèi)臟、血管壁,橫紋肌,橫紋肌,無(wú)橫紋,收縮,隨意肌 快 不持久,不隨意肌 有節(jié)律 持久,不隨意肌 慢 持久,(1)Skeletal muscle 骨骼肌 HE NO.21

3、,Naked eye: The section of tongue is pink color. 肉眼：舌頭切面，染成粉紅色 Lowest power objective lens 4: The most tissue is stained in pink. 低倍鏡4*：表面凹凸不平，深藍(lán)色的為舌黏膜上皮，深面為骨骼肌，大部分染成粉紅色。,Lower power objective lens 10:Move slide to find various sections (longitudinal, oblique and cross section) of skeletal muscle fi

4、bers. 低倍鏡10*：縱、斜、橫切面,Skeletal muscle fibers (lower magnification),Cross section,Longitudinal section,Blood vessel (oblique section),Lower power objective lens 10: This is longitudinal section of skeletal muscle fibers. Compare the staining of muscle tissue with LCT. What are their differences? 低倍鏡10

5、*：骨骼肌的縱切面。 試比較其與疏松結(jié)締組織的不同。,Skeletal muscle fibers (longitudinal section, lower magnification),(skeletal muscle fibers),loose connective tissue,High power objective lens 40: Analyses the structure in different sections of individual skeletal muscle fiber. Multi nuclei are peripheral location just und

6、erneath of the sarcolemma, which can be seen in various sections.,(the nucleus of skeletal muscle fiber),(skeletal muscle fiber),a skeletal muscle fiber(cross section),myofibrils(cross section),myofibrils(longitudinal section),loose connective tissue,高倍鏡40*：分析不同切面的骨骼肌特征。,多核，位于肌纖維的周邊，緊靠肌膜內(nèi)面，不同切面均可見。,

7、High power objective lens 40: In the longitudinal of skeletal muscle fiber the myofibrils are very thin parallel with the length of the cell. And identify its cross alternative light and dark band (cross stria) with the diaphragm closed down. 40*鏡縱切面：與長(zhǎng)軸平行的細(xì)絲為肌原纖維。縮小光圈區(qū)分明暗相間的橫紋-明暗帶。,(the nucleus of

8、skeletal muscle fiber),(skeletal muscle fiber),(loose connective tissue),cross stria,High power objective lens 40: In the cross section of skeletal muscle fiber the myofibrils are stippled (dot) appearance. The delicate LCT surrounding each skeletal muscle fiber can be seen, which is termed endomysi

9、um. 40*橫切面：肌原纖維呈細(xì)點(diǎn)狀結(jié)構(gòu)，周圍包繞的細(xì)致疏松結(jié)締組織為肌內(nèi)膜。,a skeletal muscle fiber (cross section),myofibrils (cross section),myofibrils (longitudinal section),繪圖格式,圖名， 染色方法，放大倍數(shù),圖題,？,？,？,？,？,標(biāo)出骨骼肌某些典型結(jié)構(gòu)的名稱,用簡(jiǎn)單的話描述其結(jié)構(gòu)特征,Special stain: In order to easily define muscle tissue with CT, using this special stain to recogn

10、ize them. In this staining the muscle tissue is in red, CT is in yellow. 特殊染色（熒光桃紅）： 肌組織紅色，結(jié)締組織黃色。,Special stain:,Skeletal muscle fibers (red) Loose connective tissue (yellow),(2) Cardiac muscle心肌 HE NO.19; special stain, NO.20,Naked eye: The section of the wall of heart is pink color. 肉眼：心臟切面染成粉紅色。

11、 Lowest power objective lens 4: Move the slide you will find various sections of cardiac muscle fibers. 低倍鏡4*：找到不同切面的心肌纖維。,通過(guò)比較觀察心肌的形態(tài)結(jié)構(gòu)（光鏡）,肌纖維整體形態(tài) 肌漿（胞漿） 胞核 有無(wú)特殊結(jié)構(gòu) 肌纖維間的結(jié)締組織,Lower power objective lens 10: To identify various sections of the cardiac muscle fibers. Among the cardiac muscle fibers th

12、ere is less amount of LCT and rich capillaries. Look for the perfect cross and longitudinal section of cardiac muscle fibers. Then turn to Lens 40 . 10*不同切面。肌纖維之間有少量疏松結(jié)締組織和毛細(xì)血管。找到典型的橫、縱切面轉(zhuǎn)到高倍鏡下觀察。,blood vessle,blood vessle,Loose connective tissue,cross section,Cardiac muscle (low magnification),long

13、itudinal section,High power objective lens 40: Compare to the skeletal muscle fibers. In the longitudinal of cardiac muscle fibers, which are branching and anastomosing with others, the cross-striated banding pattern is visible but not so clear than that of skeletal muscle fibers. The nucleus is loc

14、ated in the center, and surrounding by light cytoplasm due to without myofibrils. Try to find intercalated disc, which is darkly stained straight or step-like line crossing the fibers at interval.,Cardiac muscle fibers (longitudinal section-high magnification),intercalated disc,The nucleus of cardia

15、c muscle fiber,cross-striated band,blood vessle,40*縱切面：心肌纖維呈短柱狀，有分叉，互相吻合。明暗相間的橫紋不如骨骼肌明顯。核呈卵圓形，位于細(xì)胞中央，兩端無(wú)肌原纖維，被淺染的胞漿包繞。在心肌纖維連接處染色較深的結(jié)構(gòu)為閏盤，呈直線或者梯形。,High power objective lens 40: In the cross section of cardiac muscle fibers, they are different size and appearance, the large and round appearance with o

16、r without centrally nucleus, the large and pear-like appearance with or without centrally nucleus, and the small and round shaped only with stippled appearance myofibrils.,loose connective tissue,blood vessel,a cardiac muscle fiber (cross section),40*橫切面：肌纖維呈大小不一的塊狀。細(xì)胞核位于中央，核周圍淡染。肌纖維切面有的比較大，圓形或梨形，伴或

17、不伴細(xì)胞核，有的比較小，呈圓形，僅為斑點(diǎn)狀的肌原纖維。,Special stain: In section NO.20, the intercalated disc is shown clearly. 特殊染色能清楚地顯示閏盤。,Intercalated disc,fibroblast,the nucleus of cardiac muscle fiber,(3) Smooth muscle平滑肌 HE NO.23,Naked eye: The section of the intestine is pink color. 肉眼：小腸切片，染成粉紅色。 Lowest power objecti

18、ve lens 4: Look for the smooth muscle in pink color. 低倍鏡4*：找到染成粉紅色的平滑肌。,Lower power objective lens 10:Move slide to find various sections (longitudinal, oblique and cross section) of smooth muscle fibers. 低倍鏡10*：不同切面的平滑?。v橫斜切面）。,Longitudinal section,loose connective tissue,oblique section,Smooth muscle (low magnification),High power objective lens 40: In longitudinal section, The smooth muscle cell is spindle-shaped with an elongated centrally located nucleus. The narrow part of one cell lied adjacent to the broad parts of