PAGEPAGE8醫(yī)學(xué)范文內(nèi)容內(nèi)容內(nèi)容內(nèi)容摘要,論文內(nèi)容內(nèi)容內(nèi)容內(nèi)容摘要與提綱〔-〕-Epigallocatechingallate〔EGCG〕asoneofestercatechins,whichisabioactivemolecule,hasbeenshowntopossessvarietiesofhealthfunctionssuchasantioxidant,anti-ultravioletradiationandprotectionagainstskincancer,etc.However,thestabilityofEGCGunderhumanpHenvironmentispoortocausealowbioavailability.Therefore,howtoenhancebioavailabilityofEGCGisahotissueatpresentTheethosomeisanovelcarrierforenhanceddeliveryinskin,whichhasbeenreportedtohaveproductiveadvantages,suchashighentrappingcapacityandexcellentskinpermeation.Itispotentialtoimprovestabilityandbioavailabilityofdrugs,ifdrugsareentrappedbyethosomes.Inthepresentstudy,preparationmethodofnano~ethosomesentrappingEGCGwasinvestigatedanditsimprovedeffectonanti-ultravioletradiationwaschecked.〔1〕Theconditionsofpreparingnano-EGCGethosomeswereinvestigated.Byacombinationofinjectingandultrasonicemulsificating,nano-ethosomesentrappingEGCGweresuccessfullyprepared.Theoptimumparametersforthepreparationwereobtainedbytheorthogonaltest.Theresultsshowedthat2%phospholipidsand30%ethanolforformationofethosomes,0.1%K9and1.0%Tween-80asstabilizers,and0.25%EGCGasdrugunderultrasonicprocessingfor2mincouldyieldexcellentethosomeswithmeansize62.8nm,zetapotential-42.8mVandPDI〔polydisperantindex〕0.097.Theentrappingefficiencyofthenanoethosomeswasmorethan90%.〔2〕Skinpermeationexperimentswereconductedbycnfocallaserscanningmicroscope〔CLSM〕usingrodmineBasafluorescenceprobe.Theresultsshowedthatintermsofthetransdermaldepthofethosomes,theEGCGnano-ethosomescouldpermeatedeeperthantheEGCGhydroethanolicsolution,andtheEGCGtransdermalquantitybyethosomeswasobviouslygreaterthanthatofthehydroethanolicsolution.ThetransdermaldeliveryinvitrowasstudiedbymeasuratingtheamountofEGCGinorthroughskin.FortheEGCGnano-ethosomestheEGCGamountcouldreach40.13jig?cm2and289.8路？cm2at8hand24htransdermalpenetrationwhilethoseoftheEGCGhydroethanolicsolutionwereonly18.26jxg?cm2and35.67〔ig?cm*2.〔3〕Theanti-ultraviolet-B〔UVB〕irradiationwastestedbyanimalerxperimentwithnudemice.AfterthemiceweregivenUVBirradiationwithatotaldoseof625mJ/cm2,theskintissuechangewasobsveredbylightmicroscopeandTEM.TheresultsdemonslratedthatEGCGnano-ethosomescouldattenuateUVB-inducedskindamage,anditseffectwasclearlybetterthanEGCGhydroethanolicsolution.ABSTRACTInthestatisticalexperiment,subjectsmayexperiencetheoutcomeofinterestmorethanonce,theeventarecalledrecurrenteventandtheobservationoftheseoutcomesarecalledrepeatedeventsdataorrecurrenteventdata.Thistypeofdataarisesinmanyfieldssuchasmedicalfield,publichealth,biology,demographyandeconomics.Toanalyzerecurrenteventdata,variousmodelsandapproacheshavebeenproposedintheliterature.Theresearchesofrecurrenteventsincludethenumberofeventsuptotheobservationtime,theeventtimesandtheinter-eventtimes〔gaps〕。Themodelsandapproachesforthenumberofeventsovertimeswerestudiedbymanyauthors.Generally,lowdimensionalcovariateshavebeenassumedinmodelingrecurrenteventdata,butwhichmaybeviolatedintheanalysiswhenthecovariatesarehigh-dimensional.Inrecentyears,withthedevelopmentofcomputersandotherinformationtechnology,thedataandthecovariatesaremorecomplexfromthestudiesofbiology,medicine,ecology,demography,environmentandeconomicsandotherdisciplines.Someusefulimportantinformationisoftenhiddenbehindthehigh-dimensionaldata.So,recently,thehigh-dimensionaldataregressionisverypopularinmanyareas.Duetothedimensioncurseexists,manytraditionalstatisticalmethodshaveencounteredbigchallengesandunprecedenteddifficulties.Thus,inourpaper,wediscussthedimensionreductionapproachesonthejointmodelofrecurrenteventandterminaleventwithhigh-dimensionalcovariatestosolvethedimensionreductionproblemsinrecurrenteventmodel.Inourpaper,themodelwediscussedisthejointmodelofthemultiplicativehazardratefunctionofrecurrenteventandterminationevent.Theinnovationofthispaperisthatweusethepartialsufficientdimensionreductiontheoryandmethodonthejointmodel.Wenotonlystudythedimensionreductionproblemoftherecurrenteventmodel,butalsoconsiderthedimensionreductionproblemoftheterminaleventmodel.Theapplicationofthepartialsufficientdimensionreductiontheoryandmethodonrecurrenteventmodelandthedimensionreductionproblemofterminaleventmodelareworthytostudyandnottopaymoreattentionintheliterature.Inthispaper,wemainlyfocusontwoaspects:Thefirstaspect,tosolvethedimensionreductionproblemofthemultiplicativehazardratefunctionofrecurrenteventmodel,firstanonparametricestimatorisproposedforthebaselinefunction,andthebasisofthepartialcentralsubspaceanditsstructuraldimensionareestimatedthroughthepartialsufficientdimensionreduction.Basedontheestimatedstructuraldimension,thebasisofthepartialcentralsubspaceandthebaseline,wecangettheestimatoroftheregressionfunctionbyusingthelocallinearregression.Thesecondaspect,basedonthefrailtyfactorestimatedfromthemultiplicativehazardratemodeloftherecurrentevent,wediscussthepartialdimensionreductionofthemultiplicativehazardratemodelofterminalevent.Twomethodsareproposedinthispaper,thefirstmethod:weusethepartialdimensionreductiontogettheestimatorofthepartialcentralsubspaceandthenweusethelocalpartiallikelihoodfunctiontogettheestimatorsofthebaselinefunctionandtheregressionfunction.Thesecondmethod:theefficientestimationmethodisusedtogetthecentralsubspace.Forsimplicityincalculation,weassumethebaselinefunctionisknownintheterminaleventmodel.Asimulationisperformedtoconformandassessthetheoreticalfindings,andanexampleisalsodemonstratedonasetofchronicglaucomatousdiseasedata.ABSTRACTObjectiveThroughtheestablishmentofananimalmodelofacutelunginjuryinparaquatpoisonedrats,itcanbeobservedthattheeffectsofsalidroside〔SDS〕onMMP-2,TIMP-1oflungtissueinlunginjuredacuteparaquat〔PQ〕poisonedrats,aimingtoclarifythepresenceofsalidrosidesprotectiveandanti-fibrosisfunctiononlungs.Methods130adultmaleSDratswererandomlydividedinto:normalgroup〔NSgroup,10〕；PQmodelgroup〔60wererandomlydividedintosixsubgroups,each10atId,3d,7d,14d,21d,28d〕；SDSinterventiongroup〔60wererandomlydividedintosixsubgroups,each10atId,3d,7d,14d,21d,28d〕。Amongwhich,thenormalcontrolgroupisgavagedwithImLsalineonce,thePQmodelgroupandtheSDSinterventiongroupweregavagedwiththe20mg/kgPQdilutedwithImLsaline.Aafterparaquatpoisoninglh,theSDSinterventiongroup,wasgivendailyintraperitonealinjectionoflOmg/kgSalidrosideevery12hs.AsfortheNScontrolgroupandthePQmodelgroup,theywereinjectedwithsalinewithequalvolumntotheSDSgroup,onceevery12hs.ThePQmodelgroupandtheSDSinterventiongroupweretakenspecimensfromtherightlowerlungtissueunderanesthesiaanatomical,afterparaquatpoisoningId,3ds,7ds,14ds,2Ids,28ds.Partofthespecimenswasstoredinrefrigeratorat-70.CforRT-PCRandReal-timePCRtesting,andtheotherpartwasrinsedinsaline,fixedin10%formalin,dehydrated,madetransparent,dippedintowax.Andtheparaffin-embeddedtissueblocksandthe.slices,areforHEstainingandimmunohistochemicalstaining.ResultsComparedwiththenormalgroup,theratsinthePQmodelgroupandtheSDSinterventiongroupgottheearlysymptomsofacutelunginflammation,pulmonaryedemaandobviousbleeding.ChangesinpulmonaryfibrosiswereobservedafterexposuretoPQfor7daysanditcametotheclimaxat28thday;theSDSgroupgotthesimilarsymptoms,butwhicharerelativelymild.Incontrast,inthePQgroup,theMM